减少细胞引起的痕,促进脊髓损伤后的康复
David Oliveira Dias1, Hoseok Kim2, Daniel Holl1
1Department of Cell and Molecular Biology, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
Cell
|March 6, 2018
概括
在中枢神经系统 (CNS) 损伤后抑制细胞周围痕减少纤维化,增强轴突再生,并改善小鼠的感觉运动功能恢复.
科学领域:
- 神经科学
- 复原医学
- 细胞生物学
背景情况:
- 中枢神经系统 (CNS) 的损伤,如脊髓损伤,往往会导致由于轴突断裂而导致永久的功能缺陷.
- 在损伤部位形成痕组织是轴突再生的主要障碍.
- 细胞有助于痕组织的形成和纤维化.
研究的目的:
- 研究中枢神经系统损伤后痕形成中的细胞后代的作用.
- 为了确定抑制细胞周源纤维化是否可以促进轴突再生和功能恢复.
- 探索针对中枢神经系统损伤的治疗潜力.
主要方法:
- 在小鼠的脊髓损伤模型.
- 基因操纵以抑制细胞周的后代.
- 对痕组织和细胞外基质沉积的组织学分析.
- 使用特定通道追踪评估轴突再生.
- 评估传感运动功能的恢复.
- 视觉刺激以评估再生轴突的功能整合.
主要成果:
- 在受伤后抑制细胞外后代显著降低了纤维化和细胞外基质沉积.
- 观察到拉菲脊柱和皮质脊柱管轴突的再生增强.
- 治疗动物的感觉运动功能恢复得到改善.
- 经过再生的皮质脊髓轴突显示出功能整合到损伤以下的脊髓电路中.
- 在再生轴突数量和功能恢复之间发现了正相关性.
结论:
- 在中枢神经系统受伤后,减弱细胞纤维化是一种可行的策略,以促进轴突再生.
- 针对脊髓细胞提供了一个有前途的治疗途径来改善脊髓损伤的结果.
- 通过痕调节促进轴突再生可以导致显著的功能恢复.
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