在活细胞中进行电子转移成像的以纳米孔为基础的不对称放大
Yi-Lun Ying1, Yong-Xu Hu1, Rui Gao1
1Key Laboratory for Advanced Materials & School of Chemistry and Molecular Engineering , East China University of Science and Technology , Shanghai 200237 , PR China.
Journal of the American Chemical Society
|March 13, 2018
概括
研究人员开发了一种新型不对称的纳米孔电极,用于敏感的实时监测活细胞中的NADH等氧化还原活性物种. 这一突破显著放大了信号, 使细胞代谢研究和药物效应评估成为可能.
科学领域:
- 生物医学工程
- 分析化学
- 细胞生物学
背景情况:
- 实时监测细胞内电子转移,酶活性和生化信使对于了解细胞信号至关重要.
- 现有的检测活细胞中氧化还原活性物种的方法缺乏通用性和分区特异性.
- 由于3D制造的复杂性,用于细胞内检测的纳米电极制造面临着实现高信号噪声比的挑战.
研究的目的:
- 开发一种可通用的方法,以在亚细胞水平上对单个活细胞内的广泛的氧化还原活性物种进行表征.
- 报告一种基于纳米孔电极的不对称放大机制,用于实时监测活细胞中的尼古丁胺二核酸 (NADH).
- 能够对微量氧化还原活性物种进行敏感和选择性探测,并评估细胞对抗癌药物等刺激的反应.
主要方法:
- 采用两步的3D制造工艺,制造出直径小于90nm的模拟无对称纳米孔电极.
- 不对称的几何结构促进了显著的潜在下降,将法拉达电流转换为可辨别的泡诱导的短暂离子电流.
- 实现了至少3个数量级的信号放大,提高了无线检测的电流分辨率,从纳米安培升至皮卡安培.
主要成果:
- 开发的不对称纳米孔电极可实时检测活细胞中的氧化还原代谢,信号与噪声比较高.
- 该系统可实现高灵敏性和选择性探测NADH度到1皮科莫拉 (pM).
- 该电极成功监测活细胞中的呼吸链 (NADH),并评估抗癌药物对MCF-7细胞的影响.
结论:
- 集成的无线不对称纳米孔电极提供了一个有希望的平台,用于对氧化还原活性物种进行敏感的实时细胞内分析.
- 这项技术显著提升了研究活细胞中电子转移动态和细胞通信的能力.
- 该方法为未来的细胞内复杂生化过程的成像提供了基础.
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