对碳酸盐敏感的植物转化素控制藻中高亲和铁的吸收
Jeffrey B McQuaid1,2, Adam B Kustka3, Miroslav Oborník4,5
1J. Craig Venter Institute, Microbial and Environmental Genomics, La Jolla, California 92037, USA.
Nature
|March 15, 2018
概括
藻利用一种叫做ISIP2A的蛋白质吸收稀缺的铁,这个过程依赖于铁和碳酸. 海洋酸化可能会损害海洋生物的重要营养吸收机制.
科学领域:
- 海洋生物学
- 生物化学
- 海洋学
背景情况:
- 铁是巨大的海洋中植物的限制营养素.
- 藻是重要的真核浮游生物,它们需要不稳定的铁来生长.
- 在此之前,藻体对皮孔状不稳定铁的获取机制是未知的.
研究的目的:
- 阐明藻体获得不稳定的铁的机制.
- 描述ISIP2A蛋白在铁吸收中的功能.
- 研究碳酸盐离子在藻中铁的作用.
主要方法:
- 基因组启用转录组学以识别对铁有反应的基因.
- 在藻三角中进行基因删除和补充实验.
- 对海水碳酸系统进行操纵以评估离子依赖性.
主要成果:
- ISIP2A被确定为具有碳酸协调铁结合的植物转化蛋白.
- 删除ISIP2A损害了高亲和度铁的吸收,这种吸收被人体转移素恢复.
- 铁的吸收显示出对不稳定铁和碳酸盐离子度的二次依赖,表明共限.
结论:
- 在藻中,ISIP2A对于高亲和度铁的吸收至关重要,它作为植物转化蛋白起作用.
- 碳酸的可用性限制了铁的吸收,突出了与不稳定的铁的协同作用.
- 通过降低碳酸离子度,海洋酸化可能对海洋生态系统的这一关键铁获取途径产生负面影响.
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