一个单细胞RNA-seq调查人类前额叶皮层的发育格局
Suijuan Zhong1,2, Shu Zhang3, Xiaoying Fan3
1State Key Laboratory of Brain and Cognitive Science, CAS Center for Excellence in Brain Science and Intelligence Technology (Shanghai), Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
Nature
|March 15, 2018
概括
这项研究通过分析2300多个单个细胞,识别35种细胞亚型及其发育途径来绘制人类前额叶皮层的发育图. 这项研究为了解早期大脑形成和神经发育障碍提供了蓝图.
科学领域:
- 神经科学
- 发育生物学
- 基因组学
背景情况:
- 人类前额叶皮质 (PFC) 对于更高层次的认知至关重要,但它的发展是复杂的.
- 在PFC发育中的功能障碍与神经发育障碍有关.
- 在胚胎PFC中识别细胞类型和发育特征具有挑战性.
研究的目的:
- 综合地绘制人类PFC早期到中期的细胞类型和发育轨迹.
- 识别神经前代细胞的新型标记基因和发育特征.
- 了解人类PFC中神经元生成和电路形成的分子调节.
主要方法:
- 从人类PFC (妊娠8至26周) 获得超过2300个单细胞的RNA测序.
- 单细胞转录组数据分析以确定细胞亚型和发育轨迹.
- 生物信息分析以选和表征细胞群和调控信号.
主要成果:
- 在人类PFC中确定了6个主要类别的35个不同的细胞亚型.
- 用新型标记基因对神经前代细胞,包括中间前代细胞进行详细的表征.
- 激发性神经元的神经发生时间线的映射和内部神经元前体的检测.
- 发现了控制神经元生成和电路形成的内在,发育依赖的信号.
结论:
- 这项研究提供了早期和中期人类PFC发育的详细细胞和分子蓝图.
- 这项发现为神经发育障碍的细胞基础提供了洞察力.
- 这种资源有助于未来对PFC功能及其监管的研究.
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