细胞吸收黄金纳米粒子由宿主-客人相互作用触发
Jesús Mosquera1, Malou Henriksen-Lacey1, Isabel García1
1CIC biomaGUNE and Ciber-BBN , Paseo de Miramón 182 , 20014 Donostia-San Sebastián , Spain.
Journal of the American Chemical Society
|March 22, 2018
概括
研究人员开发了一种控制金纳米粒子 (AuNP) 进入细胞的方法. 负电荷的AuNP通过添加特定的阴离子来激活细胞吸收,为纳米医学提供可调节的方法.
科学领域:
- 超分子化学
- 纳米技术
- 细胞生物学
背景情况:
- 控制纳米粒子细胞吸收对于向药物输送和诊断至关重要.
- 现有的调节纳米粒子进入的方法往往缺乏特异性或对生物环境敏感.
研究的目的:
- 开发一种超分子方法,精确调节小金纳米颗粒 (AuNPs) 的细胞吸收.
- 展示主机-客户化学对纳米粒子内部化按需激活的使用.
主要方法:
- 黄金纳米颗粒 (AuNPs) 的功能化与负电荷的pyranine染料.
- 通过专门设计的阴离子共价来进行细胞吸收的现场激活.
- 使用pyranines和之间的宿主-客人识别来调节纳米粒子电荷和细胞透.
主要成果:
- 负电荷的氨酸功能化的AuNP显示出有限的细胞透.
- 增加选择性结合到pyranines的补充性子,中和负电荷并激活细胞吸收.
- 通过调整或pyranines的度来调整吸收,并且在富含蛋白质的介质中有效.
结论:
- 超分子宿主-客人相互作用提供了一种高度选择性和可逆的机制来控制纳米粒子的细胞吸收.
- 这一策略为复杂的生物系统中调节纳米粒子-细胞相互作用提供了一个多功能平台.
- 这种方法有可能在向纳米医学和诊断领域应用.
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