随机异质聚合物在外来环境中保持蛋白质功能
Brian Panganiban1, Baofu Qiao2, Tao Jiang1
1Department of Materials Science and Engineering, University of California, Berkeley, Berkeley, CA 94720, USA.
Science (New York, N.Y.)
|March 29, 2018
概括
科学家们创造了新型合成聚合物, 这一突破可以开发先进的生物材料,例如用于毒素生物修复的含酶塑料.
科学领域:
- 生物材料科学
- 聚合物化学
- 生物技术
背景情况:
- 将活性蛋白纳入合成聚合物提供了新生物材料的潜力.
- 蛋白质通常在标准的聚合物加工条件下不起作用.
- 模仿内在无序的蛋白质是克服这一挑战的关键.
研究的目的:
- 设计能够在非原生环境中溶解和稳定蛋白质的合成异质聚合物.
- 为基于蛋白质的应用创造一种新的生物系统与合成材料的接口策略.
主要方法:
- 分析蛋白质序列趋势和表面化学模式.
- 设计具有优化组成和统计单体分布的四个单体随机异质聚合物.
- 在膜蛋白和含酶塑料的无细胞合成中应用异聚合物.
主要成果:
- 开发出模仿内在无序蛋白质的异质聚合物,使蛋白质在非原生条件下稳定.
- 在无细胞合成的膜蛋白中实现了适当的蛋白质折叠.
- 使用含有酶的塑料证明了毒素的成功生物修复.
结论:
- 控制异质聚合物中的统计单体分布是一种可行的蛋白质稳定策略.
- 这种方法使得基于蛋白质的功能生物材料可以用于各种应用.
- 这些发现为生物功能与合成材料的交互开辟了新的途径.
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