在线虫C. elegans中识别基于微管的细胞质电机
R J Lye1, M E Porter, J M Scholey
1Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder 80309.
Cell
|October 23, 1987
概括
研究人员在C. elegans中发现了一种新的微管结合蛋白,具有dynein和kinesin的特征. 这种独特的运动蛋白表现出运动活动和ATPase功能,表明一种新的分子运动类.
科学领域:
- 细胞生物学 细胞生物学
- 分子电机分子电机
- 生物化学 生物化学
背景情况:
- 微管是细胞骨的重要组成部分,参与细胞内运输.
- 运动蛋白质,如丁氨酸和氨酸,促进沿微管的运动.
- 了解新型运动蛋白是阐明细胞机制的关键.
研究的目的:
- 描述C. elegans.中一种新的微管结合蛋白的特征.
- 为了研究这种蛋白质的生物化学和功能性质.
- 为了确定它与已知的运动蛋白的关系.
主要方法:
- 生化分析测量MgATPase活性.
- 使用糖分梯度和DEAE Sephadex染色学进行化研究.
- 在ATP和vanadate的存在下,在紫外线下分析蛋白质裂变.
- 微管和轴膜运动性测试.
主要成果:
- 这种蛋白质具有MgATPase活性,被瓦纳达酸,N-乙基马利胺和AMP-PNP抑制,类似于dynein.
- 一个400kDa的多成分被紫外线光与ATP和vanadate分裂.
- 该蛋白质调解了依赖ATP的微管和轴膜转位,并具有"加"末尾尾,一种类似于kinesin的功能.
- 移动性是由瓦纳达酸,N-乙基马莱胺,ATP-马-S和ATP-瓦纳达酸-紫外线裂变抑制的,这使其与kinesin区别开来.
结论:
- 鉴定出来的蛋白质是一种新型的微管转位器.
- 它表现出一种独特的dynein-like和kinesin-like特性组合.
- 这一发现扩大了已知的微管子运动蛋白质的范围.
相关概念视频
Microtubules
There are three types of cytoskeletal structures in eukaryotic cells—microfilaments, intermediate filaments, and microtubules. With a diameter of about 25 nm, microtubules are the thickest of these fibers. Microtubules carry out a variety of functions that include cell structure and support, transport of organelles, cell motility (movement), and the separation of chromosomes during cell division.Microtubules are hollow tubes whose walls are made up of globular tubulin proteins. Each tubulin...
Microtubules
Microtubules are the thickest cytoskeletal filaments with a diameter of 25 nm. In prokaryotic organisms, microtubules are commonly found in locomotory appendages like cilia and flagella. In eukaryotic cells, microtubules form specialized extensions for moving fluid over the surface, like those found in cells lining the intestine.
Microtubules have two structurally similar globular protein subunits: α and β tubulins. In the cytosol, the α and β tubulins form a heterodimer. These αβ-heterodimers...
Microtubules have two structurally similar globular protein subunits: α and β tubulins. In the cytosol, the α and β tubulins form a heterodimer. These αβ-heterodimers...
Microtubule Associated Motor Proteins
Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular cargos...
The Movement of Organelles and Vesicles
In eukaryotic cells, cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
Microtubules in Cell Motility
Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
Microtubules in Cell Motility
Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...


