在神经胞的专门细胞中重复DNA的重新排列
E U Selker1, E B Cambareri, B C Jensen
1Institute of Molecular Biology, University of Oregon, Eugene 97403.
Cell
|December 4, 1987
概括
在Neurospora crassa中引入DNA导致其性周期期间的序列不稳定. 一个新的过程,即预微生物诱导的重排序 (RIP),在半变化之前重新排列现有的DNA序列,可能推动进化.
科学领域:
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 在Neurospora crassa中引入外来DNA可以破坏基因组的稳定性.
- 了解序列不稳定性对于遗传操纵和进化研究至关重要.
研究的目的:
- 为了研究DNA引入后Neurospora crassa的序列不稳定的机制.
- 为了确定性周期期间DNA重组的时间和性质.
主要方法:
- 用含有宿主,删除和异物序列的等离子体转化Neurospora crassa.
- 用遗传交叉和四级分析分析后代的序列重排的分析.
- 在重新排列的序列中研究DNA甲基化模式.
主要成果:
- 已经存在于宿主基因组中的序列在转化和交叉后被高度重新排列.
- 在复制的等离子体衍生和宿主衍生元素中都发生了重新排列.
- 独特的序列保持不变,而重新排列的序列显示出新的细胞因子甲基化.
- 四级分析表明,重组发生在化前,在受精和卡里奥加米之间.
结论:
- 在Neurospora crassa.中发现了一种新型的遗传过程,即预微生物诱导的重组 (RIP).
- RIP有助于遗传多样性,并可能在维持基因组组织方面发挥作用.
- 这一过程突出了基因组进化的独特机制,以应对DNA引入.
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