特定路径酶表达的进化趋同
Jean-Benoît Lalanne1, James C Taggart2, Monica S Guo2
1Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Physics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Cell
|April 3, 2018
概括
生物途径需要精确的蛋白质量, 即使基因调节以不同的方式发展. 这项研究揭示了多种细菌和酵母中保存的蛋白质固态度,突出了途径构建的基本原则.
科学领域:
- 分子生物学
- 进化生物学
- 系统生物学
背景情况:
- 基因调节传统上假定共表达的蛋白质也会受到类似的调节.
- 对协同调节通路的精确蛋白质固体测量的必要性在很大程度上尚未被探索.
- 了解蛋白质丰富度调整对于理解生物通路组合至关重要.
研究的目的:
- 在广的进化时间尺度上研究细菌途径中的蛋白质固体测量和调节策略.
- 为了确定尽管基因架构不同,但蛋白质的保存比例是否保持.
- 在真核生物中探索路径表达的融合进化.
主要方法:
- 对不同细菌 (0.6-2亿年) 中的蛋白质固体测量和操作结构进行大规模分析.
- 使用终端丰富的RNA测序 (Rend-seq) 来确定单核酸分辨率的转录丰度.
- 在酵母中对细菌通路和功能相似的通路进行比较分析.
主要成果:
- 在保存的细菌路径中观察到的转录丰度和基因群架构的显著分歧.
- 后转录机制补偿调控差异,保持优先的蛋白质合成速率.
- 酵母中的独立进化途径汇聚在类似的内途表达模式上.
结论:
- 无论监管策略如何,对于生物通路来说,都需要精确的蛋白质固体测量.
- 这一发现为构建生物途径提供了一个基本原则,适用于自然系统和合成生物学.
- 进化有利于确保通路功能的精确蛋白质比例的机制.
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