"呼吸"聚合体的反诱导时间控制 创建自适应纳米反应器
Hailong Che1, Shoupeng Cao1, Jan C M van Hest1
1Bio-organic Chemistry , Eindhoven University of Technology , P.O. Box 513 (STO 3.41), 5600MB Eindhoven , The Netherlands.
Journal of the American Chemical Society
|April 5, 2018
概括
研究人员开发了自调节的聚合物纳米反应器, 这种可编程生物催化剂为可适应的人造器官提供了一个新的平台.
科学领域:
- 生物材料科学
- 纳米技术
- 化学工程
背景情况:
- 聚合体是多功能纳米结构,在药物输送和催化中具有潜在的应用.
- 控制纳米反应器内的酶活性对于开发复杂的人工系统至关重要.
- 在合成生物学中,对纳米反应器功能的动态控制仍然是一个挑战.
研究的目的:
- 开发能够进行时间控制的聚合物纳米反应器.
- 研究化学燃料用于纳米反应器活动的动态切换.
- 探索这些系统作为人工器官的潜力.
主要方法:
- 合成pH敏感的聚合物,封装的过氧化酶 (HRP) 和尿素酶.
- 使用酸性尿素溶液作为化学燃料来诱导聚合物胀和透性变化.
- 监测HRP酶活性的暂时开启/关闭,以应对添加和耗尽燃料.
主要成果:
- 聚合物纳米反应器在添加燃料时呈现短暂的尺寸增加和增强的透性.
- 用HRP进行酶催化是暂时控制的,在燃料耗尽时开启和关闭.
- 这些纳米反应器在持续的燃料供应下显示了催化活动的循环重新启动.
结论:
- 自主调节的聚合物纳米反应器为反诱导的生物催化时间控制提供了一个新的平台.
- 这些不平衡系统为先进的功能材料和自适应的人造器官提供了基础.
- 开发的系统可以精确控制催化活性,模仿生物适应性.
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