从多能干细胞培养的人类心脏组织的高级成熟
Kacey Ronaldson-Bouchard1, Stephen P Ma1, Keith Yeager1
1Laboratory for Stem Cells and Tissue Engineering, Department of Biomedical Engineering, Columbia University, New York, NY, USA.
Nature
|April 6, 2018
概括
人类诱导的多能干细胞衍生的心脏组织在身体条件下迅速成熟,使患者特定的疾病模型成为可能. 这些工程心脏组织表现出类似成年人的特征,用于研究心脏发育和疾病.
科学领域:
- 心血管生物学 心血管生物学
- 干细胞生物学 干细胞生物学
- 生物医学工程 生物医学工程
背景情况:
- 人类诱导的多能干细胞 (iPSCs) 产生患者特有的心脏组织,用于疾病建模.
- 目前的iPSC衍生的心脏模型受到细胞不成熟的限制,降低了预测能力.
- 发育成熟的心脏组织对于准确的生理和疾病研究至关重要.
研究的目的:
- 为了克服iPSC衍生的心脏组织中不成熟的局限性.
- 建立一种快速成熟的iPSC衍生的心肌细胞的方法.
- 为特定患者的心脏生理学和疾病创建预测模型.
主要方法:
- 从早期的iPSC衍生的心肌细胞形成心脏组织.
- 通过逐渐增强的强度对组织进行物理调节.
- 培养组织四周,以评估成熟度.
主要成果:
- 在四周内实现了类似成人的基因表达特征和有组织的超结构.
- 证明了生理上的肉体长度,线粒体密度和横向管道.
- 呈现氧化代谢,功能性处理,以及正力-频率关系.
- 观察到,机电性质的成熟速度比其他特性慢.
结论:
- 身体修养加速了iPSC衍生的心脏组织的成熟.
- 成熟的iPSC衍生的心脏组织适合研究心脏发育和疾病.
- 这种方法提高了患者特异性心脏模型的预测能力.
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