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内皮C型尿酸对细胞起作用,调节微循环流和血压
Katarina Špiranec1, Wen Chen1, Franziska Werner1
1Institute of Physiology, University of Würzburg and Comprehensive Heart Failure Center, University Hospital Würzburg, Germany (K. Špiranec, W.C., S.C., F.W., T.N., F.K., P.E.-N., K. Schuh, M.K.).
Circulation
|April 8, 2018
概括
C型尿酸 (CNP) 调节小血管中的血液流动. 在微血管细胞中,CNP的甘酸环酶-B (GC-B) 受体对于维持正常的血管抵抗和血压至关重要.
科学领域:
- 心血管生理学
- 内分泌学
- 分子生物学
背景情况:
- 周围血管阻力显著影响动脉血压.
- 内皮C型性尿素 (CNP) 影响血管度,但其精确的细胞点仍有争议.
- 在血管光滑肌细胞 (SMC) 中发现由 CNP 激活的甘基酶-B (GC-B) 受体.
研究的目的:
- 研究GC-B受体在微血管细胞中的作用及其对CNP介导的血管扩张的影响.
- 确定微血管中的 CNP/GC-B/cGMP 信号是否能调节周围电阻和血压.
主要方法:
- 在内皮细胞 (Tie2-Cre) 或微血管SMC/pericytes (PDGF-Rβ-CreERT2) 中产生GC-B基因缺失的小鼠.
- 使用内显微镜,侵袭性/非侵袭性血液动力学和细胞周信号分析.
- 评估脏生理学,以了解对血压调节的影响.
主要成果:
- 在较小的动脉小血管和毛细血管中,CNP的血管扩张作用加剧.
- 在内皮细胞和微血管壁细胞 (pericytes) 中证实了GC- B受体表达.
- 微血管SMC和周围细胞中的GC- B损失导致了周围抵抗和高血压的升高.
结论:
- 内皮细胞核蛋白对于调节远端动脉和毛细血管血流至关重要.
- 在微血管SMC和细胞周内进行CNP-GC-B/cGMP信号传递对于维持正常的微血管抵抗和血压至关重要.
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