曼6酸盐受体和 lysosomes 的生物发生
G Griffiths1, B Hoflack, K Simons
1European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Cell
|February 12, 1988
概括
研究人员在老鼠细胞中发现了一种专门的前细胞. 这种结构对于处理内细胞通路和排序 lysosomal 蛋白质至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 曼6酸盐受体 (MPR) 对于向 lysosomal 酶至关重要.
- 了解MPRs的细胞内贩运是细胞功能的关键.
研究的目的:
- 为了研究普通大鼠细胞中曼6酸盐受体 (MPR) 的精确定位和功能.
- 描述一种涉及MPR贩运和 lysosomal酶分类的新型酸性,网状-状结构.
主要方法:
- 免疫光显微镜检测MPR局部化.
- 使用内细胞标记物 (alpha 2 macroglobulin-gold) 来研究吸收和运输.
- 对 lysosomal 酶和 lysosomal 膜糖蛋白的抗体标记 (lgp120).
主要成果:
- 在TGN,Golgi,等离子体膜和外周内基因组中发现了低水平的MPR.
- 大多数MPR局部存在于靠近高尔基河的酸性,网状状结构中.
- 这种结构富含MPR, lysosomal酶和lgp120,在37°C时接受了内细胞载荷,但不是20°C,随后将载荷转移到 lysosomes.
结论:
- 富含MPR/lgp120的结构代表了一个专门的内体 (前体),作为一个中间区间.
- 这种前酶体促进了从MPR中释放 lysosomal 酶和包装 lysosomal 蛋白质,以便传递给 lysosomes.
相关概念视频
Receptor-mediated Endocytosis
Overview
Receptor-mediated Endocytosis
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
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Delivery Pathways to the Lysosome
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Lysosomal Hydrolases
Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
Receptor Downregulation in MVBs
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
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The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
Receptor-Mediated Endocytosis
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
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