与PI结合的受体FcRIII在刺激中性粒细胞时被释放出来
T W Huizinga1, C E van der Schoot, C Jost
1Central Laboratory, Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Nature
|June 16, 1988
概括
患有阴性夜间血红蛋白尿症 (PNH) 的患者显示,由于其酸丁乙醇 (PI) 连接的缺陷,中性粒细胞上的Fc受体III (FcRIII) 水平显著降低,影响炎症反应.
科学领域:
- 免疫学 免疫学 免疫学
- 血液学 血液学 血液学
- 细胞生物学 细胞生物学
背景情况:
- 人类的细胞细胞利用Fc受体对免疫球蛋白G (IgG) 来结合免疫复合体.
- 中性粒细胞表达Fc受体II (FcRII) 和Fc受体III (FcRIII),调解细胞和激活.
- 透性夜间血红蛋白尿 (PNH) 是一种罕见的血液疾病,影响血液细胞的生产.
研究的目的:
- 调查PNH患者中性粒细胞中的Fc受体表达和功能.
- 为了确定FcRIII在中性粒细胞上的定机制.
- 探索FcRIII在炎症过程中的作用.
主要方法:
- 流细胞计量用于量化中性粒细胞上的Fc受体表达.
- 在PNH患者细胞中分析FcRIII表达.
- 使用炎症性和IgG涂层颗粒的刺激测定.
主要成果:
- PNH中性粒细胞表现出约10%的正常FcRIII水平,而FcRII表达仍然不受影响.
- 在中性粒细胞中,FcRIII被识别为酸内醇 (PI) 固的蛋白质,但不是单细胞.
- 在中性粒细胞刺激时发生FcRIII分泌,这表明在炎症中发挥了作用.
- 用IgG涂层乳珠激活中性粒细胞是正常的,这表明FcRII在这个过程中发挥了主要作用.
结论:
- PNH的特点是中性粒细胞FcRIII表达的缺陷,这与其PI-anchor的缺陷有关.
- 在炎症反应中,FcRIII脱落是潜在的机制.
- 在IgG复合体的中性粒细胞激活中,FcRII的作用比FcRIII更为重要.
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