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相关概念视频

Nuclear Transmutation03:20

Nuclear Transmutation

Nuclear transmutation is the conversion of one nuclide into another. It can occur by the radioactive decay of a nucleus, or the reaction of a nucleus with another particle. The first manmade nucleus was produced in Ernest Rutherford’s laboratory in 1919 by a transmutation reaction, the bombardment of one type of nuclei with other nuclei or with neutrons. Rutherford bombarded nitrogen-14 atoms with high-speed α particles from a natural radioactive isotope of radium and observed protons being...
Conversion of Units01:36

Conversion of Units

Sometimes, there is a need to convert from one unit to another one. For instance, reading a cookbook in which quantities are expressed in units of liters or ounces may require conversion of quantities to cups. Or, when looking up directions on how to get to a location, we may be interested to know how many miles we are going to walk. In this case, we would have to convert units of feet or meters to miles.
The first step in the unit conversion is to list the given units and the units required...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Transformers in Distribution System01:27

Transformers in Distribution System

Transformers in distribution systems can be broadly categorized into distribution substation transformers and other distribution transformers. They are crucial for stepping down high transmission voltages to levels suitable for distribution and end-user applications.
Distribution substation transformers come in various ratings and typically use mineral oil for insulation and cooling. To prevent moisture and air from entering the oil, some transformers use an inert gas like nitrogen to fill the...
Transformers with Off-Nominal Turns Ratios01:25

Transformers with Off-Nominal Turns Ratios

In scenarios involving parallel transformers with disparate ratings, developing per-unit models requires accommodating off-nominal turns ratios. This situation arises when the selected base voltages are not proportional to the transformer’s voltage ratings. Consider a transformer where the rated voltages are related by the term a. If the chosen voltage bases satisfy a relationship involving term b, term c is defined as the ratio of these bases. This ratio is then substituted into the rated...

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相关实验视频

Updated: Jun 24, 2026

Capture Compound Mass Spectrometry - A Powerful Tool to Identify Novel c-di-GMP Effector Proteins
12:11

Capture Compound Mass Spectrometry - A Powerful Tool to Identify Novel c-di-GMP Effector Proteins

Published on: March 29, 2015

将lambda Cro转化为一个转录激活器.

F D Bushman1, M Ptashne

  • 1Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.

Cell
|July 15, 1988
PubMed
概括
此摘要是机器生成的。

兰巴达抑制剂通过与RNA聚合酶相互作用来激活转录. 研究人员将这种"激活贴片"转移到Cro蛋白中,创建了一个新的转录激活器,证明其足够激活.

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Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
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Laboratory Scale Slow Cook-Off Testing of Rocket Propellants: The Combustion Rate Analysis of a Slowly Heated Propellant (CRASH-P) Test
06:52

Laboratory Scale Slow Cook-Off Testing of Rocket Propellants: The Combustion Rate Analysis of a Slowly Heated Propellant (CRASH-P) Test

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09:24

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

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Laboratory Scale Slow Cook-Off Testing of Rocket Propellants: The Combustion Rate Analysis of a Slowly Heated Propellant (CRASH-P) Test
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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物化学 生物化学

背景情况:

  • 兰巴达抑制剂与DNA结合,通过与RNA聚合酶相互作用刺激转录.
  • 突变分析发现了对转录激活至关重要的兰巴抑制器上的酸性补丁.

研究的目的:

  • 为了确定兰巴抑制剂的确定的"激活贴片"是否足以进行转录激活.
  • 为了研究这种激活贴在其他蛋白质和菌体中的作用.

主要方法:

  • 隔离了在转录激活中受损的兰巴抑制剂突变.
  • 将"激活贴片"从兰巴抑制剂转移到兰巴克罗蛋白.
  • 分析修改后的Cro蛋白的转录活性.

主要成果:

  • 兰巴达抑制剂的"激活贴片"被发现足以在新的序列背景下进行转录激活.
  • 修改的兰巴克罗蛋白,含有抑制器的激活贴片,表现出转录激活器特性.
  • 有证据表明,菌体434抑制剂利用类似的激活贴片进行转录.

结论:

  • 兰巴达抑制器上的酸性"激活贴片"是转录激活的关键功能域.
  • 这种激活贴片可以赋予其他蛋白质转录激活能力.
  • 涉及类似激活补丁的转录激活的保存机制可能存在于不同的菌体抑制剂中.