开发一系列实用的光化学工具,用于测量活样本的pH值
Shodai Takahashi, Yu Kagami, Kenjiro Hanaoka
1Graduate School of Science and Engineering , Saitama University , 255 Okubo, Sakura-ku , Saitama-shi , Saitama 338-8570 , Japan.
Journal of the American Chemical Society
|April 25, 2018
概括
研究人员使用不对称的胺支架开发了新的光pH探测器. 这些探测器能够精确地绘制细胞内和细胞外的pH值,进步蛋白质贩运,功能衰竭和癌症研究.
科学领域:
- 化学生物学
- 生物医学成像
- 光光谱学
背景情况:
- 精确的pH调节在生物系统中至关重要,影响蛋白质降解,细胞内流通,功能衰竭和癌症等过程.
- 目前用于pH成像的比度光探针存在一些局限性,包括不合适的pKa值,较差的光漂白电阻,以及在生理条件下低于最佳的激发/发射波长.
研究的目的:
- 开发基于不对称的罗达胺的多功能杆,用于比度光pH探针.
- 创建新的pH探测器以改善生物应用的特性.
- 证明这些探针在生物系统中成像细胞内和细胞外pH的实用性.
主要方法:
- 设计和合成用于pH探测的新型不对称罗达胺支架.
- 开发了两种特定的探针:SiRpH5用于酸性细胞内,Me-pEPPR是一种近红外 (NIR) 探针用于细胞外pH.
- 使用标记生物分子 (转激素和右激素) 的探针用于蛋白质贩运的时间间隔成像和管和瘤的现场成像.
主要成果:
- SiRpH5 显示适合 pKa 和水溶性用于图像酸性细胞内区间,使得在蛋白质流通过程中首次时间间隔图像pH.
- 一个NIR探测器Me-pEPPR在管和瘤中成功成像了细胞外pH值.
- 开发的探测器克服了现有的pH成像工具的局限性.
结论:
- 不对称的罗达胺支架为创建先进的比度光pH探针提供了多功能平台.
- 这些新型化学工具对于研究细胞内和细胞外pH对生物过程的影响是有价值的.
- 这些探测器有助于体内成像,并为了解与pH相关的疾病和生物事件提供了新的可能性.
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