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相关概念视频

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Viral Recombination00:57

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Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
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Viral Structure00:56

Viral Structure

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Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
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Viral Mutations00:36

Viral Mutations

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A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
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Diagnosing and managing appendicitis requires a structured and comprehensive approach that spans from initial assessment to postoperative care. Here is an overview of the process:
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相关实验视频

Updated: Feb 11, 2026

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

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使用CRISPR-Cas13进行现场部署的病毒诊断

Cameron Myhrvold1,2, Catherine A Freije1,2,3, Jonathan S Gootenberg4,5,6,7,8

  • 1Broad Institute of the Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA. pardis@broadinstitute.org cmyhrvol@broadinstitute.org cfreije@broadinstitute.org.

Science (New York, N.Y.)
|April 28, 2018
PubMed
概括
此摘要是机器生成的。

一个新的诊断平台,SHERLOCK (特定高灵敏度酶记者解锁),可以快速检测患者样本中的寨卡病毒 (ZIKV) 和登革热病毒 (DENV). 通过HUDSON (加热未提取的诊断样本以消灭核酶) 协议,可以在不到两个小时的时间内进行无仪器检测.

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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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科学领域:

  • 分子生物学
  • 病毒学
  • 生物技术

背景情况:

  • 全球传染病缓解需要敏感,具体和现场部署的诊断工具.
  • 现有的诊断方法可能缺乏迅速应对疫情所需的速度和可访问性.

研究的目的:

  • 评估基于Cas13的SHERLOCK平台对寨卡病毒 (ZIKV) 和登革热病毒 (DENV) 的敏感和特异性检测.
  • 开发和验证HUDSON协议,直接从患者样本中快速检测病毒.
  • 评估SHERLOCK在区分DENV血清型和ZIKV菌株方面的能力.

主要方法:

  • 使用基于Cas13的Sherlock平台进行核酸检测.
  • 开发并实施HUDSON准备样本的协议.
  • 对ZIKV和DENV的测试灵敏度降至每微升1副本.
  • 证实了谢洛克能够区分DENV血清型和ZIKV菌株.

主要成果:

  • SHERLOCK检测到ZIKV和DENV的度只有1副本/μL.
  • 通过HUDSON协议,可以在不到2小时的时间内从患者样本中检测DENV.
  • 谢洛克成功区分了四种DENV血清和流行性ZIKV菌株.
  • 实现了快速 (< 1 周) 设计和测试病毒单核酸多态性的无仪器测试.

结论:

  • SHERLOCK平台与HUDSON协议相结合,为ZIKV和DENV提供了灵敏,具体和快速的诊断解决方案.
  • 这种方法有助于无仪器检测,这对于管理传染病爆发至关重要.
  • 该平台能够检测特定的病毒变异和单核酸多态性,从而提高其在分子流行病学中的实用性.