动态共价结合基因的菌体显示使得针对性的抗生素易于开发
Kelly A McCarthy1, Michael A Kelly1, Kaicheng Li1
1Department of Chemistry, Merkert Chemistry Center , Boston College , Chestnut Hill , Massachusetts 02467 , United States.
Journal of the American Chemical Society
|April 28, 2018
概括
一个新的菌体显示平台快速识别特定细菌菌株的质探针,使得针对性的抗生素开发和诊断能够对抗抗生素耐药性的上升. 这种方法有助于制造对抗抗性病原体的窄谱治疗方法,如金黄色葡萄球菌和宝曼菌.
科学领域:
- 微生物学
- 生物技术
- 药物发现
背景情况:
- 细菌病原体的抗生素耐药性是一个重大的全球健康威胁.
- 过度使用广泛的抗生素有助于抗药性,
- 需要使用狭窄光谱的药物来消除病原体,同时保留有益的开始性细菌.
研究的目的:
- 开发一种菌体显示平台,以快速识别针对细菌菌株的特定探针.
- 为开发特定菌株的杀菌剂创造一种方法.
- 应对对抗性细菌的新型诊断工具和抗生素的迫切需要.
主要方法:
- 采用菌体显示库,其中包含2甲酸,用于对细菌细胞表面的动态共价结合.
- 对菌体库进行查,包括黄金葡萄球菌和宝曼菌的临床菌株.
- 将已识别的探头转化为特定的毒素的杀菌剂.
主要成果:
- 对抗生素耐药的金黄色葡萄球菌和宝曼菌菌株的已识别的亚微分子和高度特异性结体.
- 证明探针成功转化为向的杀菌剂.
- 验证了该平台对各种细菌菌株的适用性.
结论:
- 描述的菌体显示平台有助于快速识别特定的细菌向.
- 这项技术可以开发特定菌株的抗生素和诊断工具.
- 提供了对抗抗生素耐药性和改善感染治疗的有希望的策略.
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