转接涉及一种新型的小核核核糖核蛋白颗粒
J P Bruzik1, K Van Doren, D Hirsh
1Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510.
Nature
|October 6, 1988
概括
在三生体和线虫中进行转接,使用拼接的领导者 (SL) RNAs,形成Sm小核核核糖核蛋白颗粒 (SL snRNP). 这些SL snRNP可能会激活它们自己的5'拼接位点,从而可能消除了在跨拼接中对U1 snRNP的需求.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生物化学
背景情况:
- 转接,一个过程,涉及从一个拼接的领导RNA (SLRNA) 到前体mRNA的异构体的转移,发生在trypanosomes和Caenorhabditis elegans等生物体中.
- 这个过程与cis-splicing有相似之处,这表明细胞机械的潜在重叠.
研究的目的:
- 研究SLRNAs在转链剪接中的结构和功能特征.
- 确定SLRNAs的作用及其与细胞组件的相互作用,特别是小核核糖核蛋白颗粒 (snRNP).
主要方法:
- 在各种物种中预测SLRNA的二次结构.
- 用HeLa细胞核提取物化SLRNAs.
- 使用抗Sm抗体和其他针对哺乳动物snRNP蛋白的自身抗体进行免疫沉降测定.
主要成果:
- 来自不同物种的SL RNAs表现出保留的二次结构,具有三个茎环,尽管序列和长度的变化.
- 在SLRNA中存在一个共识的Sm结合序列.
- SL RNAs与抗Sm抗体形成免疫可接受的复合体,表明与Sm snRNP (SL snRNP) 的关联.
结论:
- 在转接过程中,SLRNAs可能会作为SL snRNP发挥作用.
- SL snRNP可能会自主激活它们自己的5'拼接位,可能会取代 cis-splicing 中看到的U1 snRNP的功能.
相关概念视频
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Nuclear Export of mRNA
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing: RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...


