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分子克隆和人类干扰素玛受体的表达

M Aguet¹, Z Dembić, G Merlin

¹Institute of Immunology and Virology, University of Zürich, Switzerland.

|October 21, 1988

概括

研究人员隔离了人类干扰素玛受体 (IFN-γR) 基因. 虽然它在小鼠细胞中类似地结合,但人类的IFN-γ敏感性需要特定物种的因素,揭示了一种新的跨膜受体.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

干扰因子- (IFN-γ) 是一种关键的细胞因子,参与免疫反应.
了解IFN-γ受体 (IFN-γR) 是阐明IFN-γ信号通路的关键.
人类的IFN-γR结构和功能尚未完全表征.

研究的目的:

隔离和描述编码人类干扰素玛受体的cDNA.
在异质系统中研究人类IFN-γR的功能表达.
为了确定人类IFN-γR基因的染色体位置.

主要方法:

使用lambda gt11表达库和多克隆抗血清分离人类IFN-γRcDNA.
在宇宙生物库中识别IFN-γR基因.
人类IFN-γR基因转移到小鼠细胞中.
对转染细胞中人类IFN-γ的结合特性和功能敏感性的分析.
对cDNA的序列分析以推断蛋白质结构和同质性.
染色体局部化研究.

主要成果:

成功分离了人类干扰素玛受体的cDNA.
感染过的小鼠细胞表达了人类的IFN-γR,具有类似人类的结合特征.
感染过的细胞对人类IFN-γ没有表现出敏感性,这表明需要特定物种的辅助因子.
人类的IFN-γR序列与已知的蛋白质没有相似之处,这表明它是一种新型的跨膜受体.
该基因很可能位于人类染色体6q上,并来自单个mRNA.
人类的IFN-γR代表了一类新型的跨膜受体.

结论:

人类的干扰素马受体是一种新型的跨膜蛋白.
特定物种的辅因子对于调解人类IFN-γ敏感性至关重要.
人类的IFN-γR基因位于6q染色体上.
需要进一步的研究来确定参与IFN-γR功能的辅因子.

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