原生电子喷射电离质谱揭示了阿普塔默-子相互作用的多个方面:从机制到结合常量
Basri Gülbakan1,2, Konstantin Barylyuk1, Petra Schneider1
1Department of Chemistry and Applied Bioscience , ETH Zürich , CH-8093 Zürich , Switzerland.
Journal of the American Chemical Society
|May 8, 2018
概括
原生电喷离子质谱法 (ESI-MS) 成功表征了体-体复合物. 这种方法揭示了结合细节,固体测量和亲缘关系,进步了对分子相互作用的理解.
科学领域:
- 生物化学
- 分析化学
- 分子生物学
背景情况:
- 胺是对各种标具有高亲和力和选择性的精选寡核酸.
- 由于结构数据和生物物理工具有限,难以理解胺与配体的相互作用.
研究的目的:
- 证明原生喷射电离质谱法 (ESI-MS) 的实用性,用于表征胺-联体复合物.
- 详细介绍特定的体及其配体之间的非共价相互作用.
主要方法:
- 原生电喷离子质谱 (ESI-MS) 是主要的技术.
- 补充方法包括离子移动性光谱 (IMS),异热定位热量计 (ITC) 和循环二极化 (CD) 光谱.
- 研究的阿胺包括腺结合阿胺 (ABA),l-阿尔金纳胺结合阿胺 (LABA) 和可卡因结合阿胺 (CBA).
主要成果:
- 原生ESI-MS成功确定了体选择性和复杂的体对.
- 该方法改进了结合模型并提供了准确的结合亲和度 (例如,ABA:Kd1 = 69.7 μM,Kd2 = 5.3 μM;LABA:Kd1 = 22.04 μM;CBA:Kd1 = 8.5 μM).
- 在ESI-MS数据中区分了特定和非特定的约束事件.
结论:
- 原生ESI-MS是一个强大的工具,用于详细描述aptamer-ligand复合体.
- 这种方法有助于更好地了解胺-连接体结合机制和亲属性.
- 这些发现有助于开发和应用基于aptamer的技术.
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