相关实验视频
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An Assay for Quantifying Protein-RNA Binding in Bacteria
Published on: June 12, 2019
循环AMP响应的DNA结合蛋白:结构基于克隆的胎盘cDNA
J P Hoeffler1, T E Meyer, Y Yun
1Laboratory of Molecular Endocrinology, Massachusetts General Hospital, Boston.
概括
研究人员确定了一种循环AMP响应元素结合蛋白 (CREB),该蛋白与调节基因转录的DNA序列结合. 这种CREB蛋白与参与细胞过程的其他转录因子具有结构上的相似性.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 细胞生物学 细胞生物学
背景情况:
- 循环AMP (cAMP) 作为一个关键的细胞内第二信使.
- cAMP调节了许多细胞基因的转录.
- 一个特定的DNA序列,TGACGTCA (cAMP-响应元素,CRE),调解了这种转录调节.
研究的目的:
- 为了分离和描述与CRE序列结合的蛋白质.
- 为了确定参与cAMP介导基因调节的特定CRE结合蛋白 (CREB).
主要方法:
- 使用放射性CRE探针对胎盘的lambda gt11表达库进行查.
- 对编码CRE结合蛋白的cDNA进行隔离和表征.
主要成果:
- 隔离了一种编码326氨基酸蛋白的cDNA,具有CRE结合性质.
- 鉴定的蛋白质CREB具有一个基本区域,与氨酸拉链状序列相邻,涉及DNA结合和蛋白质与蛋白质相互作用.
- CREB具有酸性N端区域,可能作为转录激活域.
- CREB的DNA结合域与c-jun和酵母GCN4转录因子中的域具有结构同质性.
结论:
- 一种新型的CRE结合蛋白 (CREB) 已被确定和描述.
- 克雷布在cAMP介导的基因转录中起作用.
- CREB的结构特征表明,在不同物种和途径中,DNA结合和转录调节的机制得到保护.
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