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相关概念视频

Microtubules01:35

Microtubules

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There are three types of cytoskeletal structures in eukaryotic cells—microfilaments, intermediate filaments, and microtubules. With a diameter of about 25 nm, microtubules are the thickest of these fibers. Microtubules carry out a variety of functions that include cell structure and support, transport of organelles, cell motility (movement), and the separation of chromosomes during cell division.
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Microtubules01:18

Microtubules

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Microtubules are the thickest cytoskeletal filaments with a diameter of 25 nm. In prokaryotic organisms, microtubules are commonly found in locomotory appendages like cilia and flagella. In eukaryotic cells, microtubules form specialized extensions for moving fluid over the surface, like those found in cells lining the intestine.
Microtubules have two structurally similar globular protein subunits: α and β tubulins. In the cytosol, the α and β tubulins form a heterodimer....
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The Quantum-Mechanical Model of an Atom02:45

The Quantum-Mechanical Model of an Atom

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Shortly after de Broglie published his ideas that the electron in a hydrogen atom could be better thought of as being a circular standing wave instead of a particle moving in quantized circular orbits, Erwin Schrödinger extended de Broglie’s work by deriving what is now known as the Schrödinger equation. When Schrödinger applied his equation to hydrogen-like atoms, he was able to reproduce Bohr’s expression for the energy and, thus, the Rydberg formula governing hydrogen spectra.
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The Energies of Atomic Orbitals03:21

The Energies of Atomic Orbitals

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In an atom, the negatively charged electrons are attracted to the positively charged nucleus. In a multielectron atom, electron-electron repulsions are also observed. The attractive and repulsive forces are dependent on the distance between the particles, as well as the sign and magnitude of the charges on the individual particles. When the charges on the particles are opposite, they attract each other. If both particles have the same charge, they repel each other.
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Atomic Structure01:33

Atomic Structure

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Overview
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Hybridization of Atomic Orbitals I03:24

Hybridization of Atomic Orbitals I

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The mathematical expression known as the wave function, ψ, contains information about each orbital and the wavelike properties of electrons in an isolated atom. When atoms are bound together in a molecule, the wave functions combine to produce new mathematical descriptions that have different shapes. This process of combining the wave functions for atomic orbitals is called hybridization and is mathematically accomplished by the linear combination of atomic orbitals. The new orbitals that...
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相关实验视频

Updated: Feb 10, 2026

Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells
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Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells

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微管-相互作用的近原子模型

Elizabeth H Kellogg1,2, Nisreen M A Hejab2, Simon Poepsel1

  • 1QB3 Institute and Department of Molecular and Cell Biology, University of California-Berkeley, Berkeley, CA 94720, USA.

Science (New York, N.Y.)
|May 12, 2018
PubMed
概括
此摘要是机器生成的。

蛋白通过与蛋白重复结合来稳定微管. 这种对神经元功能至关重要的相互作用被过酸化破坏, 可能导致阿尔茨海默病.

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Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues
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An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
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An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons

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相关实验视频

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Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells

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An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
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科学领域:

  • 神经科学
  • 分子生物学
  • 生物化学

背景情况:

  • 是一种轴突蛋白质,对微管稳定至关重要.
  • 过化从MT中脱离,聚合,并与阿尔茨海默病有关.
  • 目前尚不清楚tau- MT相互作用和稳定性的确切机制.

研究的目的:

  • 阐明陶相互作用的原子细节.
  • 了解如何稳定微管.
  • 研究酸化对的MT结合亲和力的影响.

主要方法:

  • 微管上tau结构的冷电子显微镜 (冷EM).
  • 计算模型生成陶相互作用的原子模型.

主要成果:

  • 的保存的管结合重复采用沿原纤维延伸的结构.
  • 这些结构稳定了管二元之间的接口.
  • 提出了一种沿着原纤维的并联重复结合模型,将管二元结合并稳定MT聚合.

结论:

  • 这项研究为tau-MT相互作用提供了原子层面的见解.
  • 这些发现解释了酸化如何影响MT结合.
  • 拟议的模型阐明了在微管稳定中的作用.