古代呼吸系统的结构
Hongjun Yu1, Chang-Hao Wu2, Gerrit J Schut2
1Cryo-EM Structural Biology Laboratory, Van Andel Research Institute, Grand Rapids, MI 49503, USA.
Cell
|May 15, 2018
概括
研究人员确定了膜结合酶 (MBH) 的结构,揭示了其与复合I的进化联系. 这种酶在极高的热量下使用独特的离子通道进行ATP合成.
科学领域:
- 生物化学
- 结构生物学
- 微生物学
背景情况:
- 膜结合酶 (MBH) 和复合I是同类的呼吸酶.
- 对它们的进化关系缺乏详细的结构理解.
研究的目的:
- 阐明来自Pyrococcus furiosus的14个子单位MBH的冷电子显微镜 (冷EM) 结构.
- 为MBH和复合体I之间的进化联系提供结构基础.
主要方法:
- 使用冷电子显微镜 (cryo-EM) 来确定MBH的结构.
- 在MBH和复合I之间进行了比较结构分析.
主要成果:
- MBH结构显示了一个类似于复合I的Q模块的膜定酶模块.
- 离子转移模块具有不同的H+和Na+转移单元.
- 在复合体I中缺少的Na+转位单元,在高温下促进的演化和Na+梯度的形成.
结论:
- 尽管有建筑上的差异,但MBH结构澄清了它与复合体I的进化关系.
- 在高热性条件下使用独特的Na+梯度机制进行ATP合成.
- 提供了MRP抗载体结构和MBH演变的见解.
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