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凝聚环使用其链来利用非拓以及拓机制来组织DNA

Madhusudhan Srinivasan¹, Johanna C Scheinost¹, Naomi J Petela¹

¹Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.

|May 15, 2018

概括

姐妹染色体凝聚力依赖于凝聚环捕获DNA. 一致性

科学领域:

分子生物学
细胞生物学
遗传学

背景情况:

姐妹染色体凝聚力对于细胞分裂过程中的染色体分离至关重要.
众所周知,凝聚体在建立和维持这种凝聚力方面发挥着核心作用.
凝聚素捕获DNA形成稳定链接的精确机制仍在研究中.

研究的目的:

研究凝聚环的链域在DNA捕获和姐妹染色体凝聚中的作用.
确定凝聚素被载入并沿着染色质转移的机制.
为了确定DNA结合和凝聚的关键残留物.

主要方法:

使用圆形微染色体来评估DNA的捕获和凝聚力.
引入了凝聚链域的突变,包括其充电光和特定的氨酸残留物的中和.
在具有突变凝聚素复合体的细胞中检查了凝聚素负荷,转位和ATP水解.

主要成果:

在小染色体和姐妹染色体凝聚力之间观察到强烈的相关性.
缺乏带正电荷的链光线的凝聚素捕获了个体DNA,但未能建立凝聚力.
凝聚链中的突变取消了DNA的捕获,而Smc-kleisin融合则没有.
在 Smc1/3 链中发生的特定 lysine 突变取消了所有凝聚体载荷,但没有影响 ATP 水解或向载荷点的招募.

结论:

凝聚素通过拓 (陷) 和非拓相互作用与染色体相互作用.
凝聚性链域对于DNA的捕获至关重要,可能通过链开放机制.
在链中由ATP水解驱动的形状变化可能会介导链加载和沿着染色质的转移.

关键词:

美国染色体凝聚一致性凝聚剂循环挤出姐妹染色体凝聚力

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