一种用于噪声抑制和命运稳定的转录后反机制
Maike M K Hansen1, Winnie Y Wen2, Elena Ingerman1
1Gladstone|UCSF Center for Cell Circuitry, Gladstone Institutes, San Francisco, CA 94158, USA.
Cell
|May 15, 2018
概括
基因表达噪声驱动细胞命运的决定, 人类免疫缺陷病毒 (HIV) 使用一种新的反回路来抑制这种噪音,使其稳定.
科学领域:
- 分子生物学
- 病毒学
- 系统生物学
背景情况:
- 生物系统使用基因表达噪声来决定细胞命运.
- 在承诺后抑制噪音的机制尚不清楚.
研究的目的:
- 研究人类免疫缺陷病毒 (HIV) 在感染后如何抑制基因表达噪声.
- 阐明噪声减弱背后的机制,以实现稳定的命运承诺.
主要方法:
- 单分子成像
- 时间间隔成像
- 非转录性负反回路的分析
主要成果:
- 艾滋病毒通过后转录拼接反循环减弱表达噪声.
- 拼接的mRNA产生蛋白质,这些蛋白质会自动消耗前体未拼接的mRNA.
- 这种自动消耗电路稳定了HIV的承诺决策.
结论:
- 一个涉及延迟拼接的新反机制抑制了艾滋病毒的噪音.
- 这种机制稳定了细胞命运的承诺,这表明在同位体信号中具有更广泛的应用.
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