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ExTzBox:活细胞成像的发光旋风
Indranil Roy, Sharan Bobbala, Jiawang Zhou
1Institute of Molecular Design and Synthesis , Tianjin University , 92 Weijin Road , Nankai District , Tianjin 300072 , P. R. China.
Journal of the American Chemical Society
|May 18, 2018
概括
研究人员开发了一种新的光探测器ExTzBox·4X,用于活细胞成像. 这种明亮的非细胞毒性探针可以有效地照亮细胞而不会造成光白或损伤,克服了探针设计的先前挑战.
科学领域:
- 化学生物学
- 分子成像
- 材料科学
背景情况:
- 开发理想的光探针用于活细胞成像需要亮度,低细胞毒性和高效的细胞传递.
- 合成光体设计面临着同时实现所有所需性能的挑战.
- 现有的探针可能表现出细胞毒性或光漂白,这限制了它们在长期活细胞研究中的有用性.
研究的目的:
- 设计和合成一种新型的光探针,用于增强亮度和降低细胞毒性的活细胞成像.
- 调查一个新的盒状杂交旋风机的光物理特性和细胞吸收.
- 通过共聚焦显微镜在活细胞成像应用中证明开发的探针的有效性.
主要方法:
- 通过连接扩展的viologen (ExBIPY) 和二甲基 thiazolothiazole (TzBIPY) 单元,合成一个盒状的混合环氧化物,ExTzBox·4X (6·4X).
- 光物理特征包括量子收益率的确定 (ΦF=1.00对于6·4Cl).
- 使用共聚焦显微镜对RAW 264.7巨细胞和活细胞成像进行细胞毒性评估.
主要成果:
- 由于其刚性结构,ExTzBox·4X (6·4Cl) 具有高量子产量,并且在高度 (100μM) 时无细胞毒性.
- 聚焦显微镜证实RAW 264.7巨细胞有效吸收64+,从而产生明亮的光.
- 探测器没有显示光漂白或破坏细胞行为,与较少光的非循环参考化合物不同.
结论:
- 刚性,盒状的旋结构对于开发活细胞成像的高光和非细胞毒性探针具有重要意义.
- 在生物研究的光探针技术中,ExTzBox·4X是一个有前途的进步.
- 开发的探测器可以有效地对细胞过程进行长期可视化,而不会损害细胞活力.
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