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由单个Legionella效应体介导的基基化机制
Anil Akturk1, David J Wasilko1, Xiaochun Wu1
1Weill Institute for Cell and Molecular Biology and Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA.
Nature
|May 26, 2018
概括
研究人员阐明了基联的全方位化机制. 这种独特的翻译后修饰涉及SdeA将ubiquitin转化为基基基 (PR-Ub) 以进行血清合.
科学领域:
- 生物化学
- 分子生物学
- 结构生物学
背景情况:
- 化是一种关键的真核转化后修饰,调节细胞过程.
- 传统的无化涉及到无素 (Ub) 的化.
- 莱吉欧内拉肺SIDE效应体家族使用独特的基化 (PR-Ub) 途径向血清残留物.
研究的目的:
- 要确定SdeA催化模块的晶体结构.
- 阐明基联泛化的分子机制.
- 调查SdeA内部不同功能单位的角色.
主要方法:
- SdeA和SdeD碎片的X射线结晶学
- 用于分析酶活性的生物化学测试.
- 酶基质复合物的结构分析.
主要成果:
- 该SdeA催化模块包括基酶和单ADP-基转移酶域.
- SdeA表现出将Ub转化为ADP-ribosylatedUb (ADPR-Ub) 和将PR-Ub结合到基板的独立活动.
- 晶体结构显示了ADPR-Ub的转化为PR-Ub和AMP,以及随后的血清合.
结论:
- 这项研究确定了基联无化的分子机制.
- 这项研究提供了一个独特的翻译后修改途径.
- 这些发现为未来的研究铺平了道路.
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