Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

From DNA to Protein03:06

From DNA to Protein

22.5K
The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
22.5K
Mechanical Protein Functions01:58

Mechanical Protein Functions

5.7K
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force. 
5.7K
Structural Protein Function01:56

Structural Protein Function

30.0K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to...
30.0K
Structural Protein Function01:56

Structural Protein Function

3.3K
3.3K
Mechanical Protein Function01:58

Mechanical Protein Function

2.5K
2.5K
DNA-only Transposons02:57

DNA-only Transposons

17.5K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
17.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Metal-Phenolic Coatings Enable Universal Design of Spherical Nucleic Acids.

Angewandte Chemie (International ed. in English)·2026
Same author

Correction to "DNA-Mediated Cellular Delivery of Functional Enzymes".

Journal of the American Chemical Society·2026
Same author

High-χ Block Copolymer Nanoreactors for the Confined Synthesis of Size-Controlled Nanoclusters.

ACS nano·2026
Same author

Programmable Stepwise Heteroepitaxial Growth of Colloidal Crystals With Different Phases.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Simplex-based model for nanoparticle grain identification in four-dimensional scanning transmission electron microscopy data.

Journal of microscopy·2026
Same author

High-entropy 1D halide perovskite piezoelectrics found by megalibrary synthesis and rapid nonlinear optical screening.

Science advances·2026
Same journal

Linker Engineering toward NIR-II Metal-Organic Framework with Maximal Emission beyond 1000 nm for Inflammatory Bowel Disease Imaging.

Journal of the American Chemical Society·2026
Same journal

Observing Kinetic Selectivity in Anthracene Photodimerization through Selective Quenching by Excited States of Proximate Rare Earth Cations.

Journal of the American Chemical Society·2026
Same journal

Sequence-Dependent Folding of Recognition-Encoded Melamine Oligomers.

Journal of the American Chemical Society·2026
Same journal

Large Thermo- and Mechanosalient Actuation via Cooperative Twist Elasticity-Induced Packing Motif Conversion.

Journal of the American Chemical Society·2026
Same journal

Discovery and Biosynthesis of Lanthipeptides Featuring an Azepinoindole Scaffold by Radical <i>S</i>-Adenosylmethionine Enzyme-Catalyzed C-C Bond Formation.

Journal of the American Chemical Society·2026
Same journal

Enantiopurity-Controlled Magnetism in a Two-Dimensional Organic-Inorganic Material.

Journal of the American Chemical Society·2026
查看所有相关文章

相关实验视频

Updated: Feb 10, 2026

Identification of Functional Protein Regions Through Chimeric Protein Construction
11:39

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

11.0K

具有DNA功能的双价蛋白

Janet R McMillan1, Chad A Mirkin1

  • 1Department of Chemistry and International Institute for Nanotechnology , Northwestern University , 2145 Sheridan Road , Evanston , Illinois 60208 , United States.

Journal of the American Chemical Society
|May 26, 2018
PubMed
概括
此摘要是机器生成的。

研究人员创造了新的DNA-蛋白质结合体, 这种DNA介导的组合形成了具有定向结合的周期性线状超结构.

更多相关视频

CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

7.7K
Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
08:26

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

14.2K

相关实验视频

Last Updated: Feb 10, 2026

Identification of Functional Protein Regions Through Chimeric Protein Construction
11:39

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

11.0K
CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

7.7K
Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
08:26

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

14.2K

科学领域:

  • 生物结合化学
  • 材料科学
  • 纳米技术

背景情况:

  • 蛋白质可以与DNA功能化,以创建自组装材料.
  • 在蛋白质组合中实现定向控制是当前方法的挑战.

研究的目的:

  • 合成和表征β-galactosidase的双价DNA结合物.
  • 通过使用定向DNA结合来证明可编程访问一个维的蛋白质材料.
  • 通过受控的蛋白质相互作用来设计新类的超结构.

主要方法:

  • 用一对寡核化物功能化βGal蛋白的合成.
  • 使用凝电泳,冷传递电子显微镜和负染色传递电子显微镜进行表征.
  • 化实验分析DNA双重稳定性和结合方式.

主要成果:

  • 已经成功合成了双价DNA蛋白合物.
  • 通过DNA杂交证明了周期性,线状蛋白质超结构的形成.
  • 观察到缩小和升高的融化过渡,证实了DNA介导的结合和设计相互作用.
  • 展示了只有两种DNA修饰的定向DNA结合.

结论:

  • 通过对蛋白质进行最小的DNA修改,可以实现定向DNA结合.
  • 这种方法可以设计可编程的单维蛋白质材料.
  • 可以实现具有控制互动的新型超结构,推进自组装技术.