相关实验视频

Updated: Jul 7, 2026

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

在大肠杆菌K-12中自我调节DNA复制基因dnaA

R E Braun, K O'Day, A Wright

|January 1, 1985

概括

大肠杆菌中的dnaA基因产物调节了自己的合成. 过度生产dnaA蛋白质会减少其自身的基因表达,而突变会增加它,表明自我调节.

科学领域:

分子生物学分子生物学
微生物学微生物学
遗传学是一个遗传学.

背景情况:

dnaA基因对于启动大肠杆菌K-12中的DNA复制是必不可少的.
在复制频率中dnaA蛋白的精确调节作用仍然不清楚.

研究的目的:

为了研究dnaA基因表达的自我调节.
确定控制dnaA合成的调控机制.

主要方法:

在体内构建和分析dnaA-lacZ转化和dnaA-trpA-lacZ转录融合.
对dnaA推广者/监管区域的删除分析.
DNAase保护测定用于确定dnaA蛋白结合点.

主要成果:

dnaA融合的表达对dnaA蛋白水平很敏感.
过度生产dnaA蛋白降低了融合表达;dnaA-突变增加了它.
DNAase保护证实dnaA蛋白与自己的促进子区域结合.

结论:

dnaA基因产物自我调节自己的合成.
该dnaA蛋白抑制了其两种促进者的转录,这表明了负反循环.

更多相关视频

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Published on: September 11, 2017

相关概念视频

Replication in Prokaryotes

Replication in Prokaryotes

Replication in Eukaryotes

Replication in Eukaryotes

Replication in Prokaryotes

Replication in Prokaryotes

Replication in Eukaryotes

Replication in Eukaryotes

Replication in Prokaryotes

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...

Replication in Eukaryotes

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...