概括
研究人员确定了一种同类于人类表皮生长因子 (EGF) 受体的多索菲拉基因段. 这一发现表明,EGF受体信号通路在物种之间起着保留作用.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 进化生物学 进化生物学
背景情况:
- 脊椎动物的基因组包含许多与SRC相关的基因,大多数SRC家族蛋白质是细胞内或膜相关的.
- 一些SRC家族成员作为增长因子的跨膜受体起作用.
- 已经确定了三种与Drosophila src相关的基因序列,同类于细胞内SRC家族成员.
研究的目的:
- 调查弱v-src-杂交Drosophila序列是否代表已知的src家族基因的同类.
- 具体来说,在这个群体内识别生长因子受体基因的潜在同类.
- 描述一种具有潜在同质性与生长因子受体的新型Drosophila基因段.
主要方法:
- 对克隆的Drosophila序列进行查,以寻找与v-src瘤基因的同质性.
- 使用v-erb-B瘤基因和人类表皮生长因子 (EGF) 受体互补DNA克隆探针进行混合化分析.
- 从已识别的Drosophila基因组段中推断氨基酸序列.
主要成果:
- 四个克隆的Drosophila序列,代表重叠的基因组段,显示了与v-erb-B瘤基因的优先杂交.
- 这部分部分的推断的氨基酸序列与人体EGF受体的激酶域具有77%的相似性.
- 同一个基因组段还包含了与人类EGF受体基因细胞外域相关的序列.
结论:
- 一个同类于人类表皮生长因子 (EGF) 受体基因的Drosophila基因组段已被确定.
- 这一发现表明Drosophila和脊椎动物的EGF受体信号通路的演化起源和潜在的功能相似性得到了保护.
- 鉴定出的基因代表了Drosophila中SRC家族的新成员,可能参与生长因子信号传递.
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