在tRNA的演化过程中对和更高层次的基因相互作用
Júlia Domingo1, Guillaume Diss1, Ben Lehner2,3,4
1Systems Biology Program, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain.
Nature
|June 1, 2018
概括
基因相互作用是高度动态的,在不同的酵母tRNA基因型之间发生积极和负面影响. 了解这种表征对于在不断发展的种群中准确的遗传预测至关重要.
科学领域:
- 遗传学与进化论
- 分子生物学
- 酵母遗传学
背景情况:
- 遗传背景显著影响突变结果.
- 双向突变相互作用有助于表型变异.
- 跨基因型的基因相互作用的稳定性基本上是未知的.
研究的目的:
- 量化基因相互作用的稳定性和动态性.
- 调查更高层次的遗传相互作用.
- 评估遗传背景对突变效应的影响.
主要方法:
- 系统地绘制了45,000多种基因相互作用.
- 对500多种密切相关的酵母tRNA基因型进行分析.
- 对对和更高阶表达的量化.
主要成果:
- 所有突变对至少在9%的基因背景中相互作用.
- 在基因型之间,相互作用经常从积极转变为消极.
- 高级的相互作用是丰富的和动态的.
- 个体突变从有害转变为有益的影响.
结论:
- 基因的相互作用是高度动态的,
- 准确的基因预测需要测量各种背景的突变效应.
- 预测模型需要更高层次的表观术语.
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