对于RNA聚合酶II的C端超化相分离机制
Huasong Lu1,2, Dan Yu2, Anders S Hansen2
1School of Pharmaceutical Sciences, Xiamen University, Xiamen, China.
Nature
|June 1, 2018
概括
研究人员在cyclin T1中发现了一个富含histidine的域,该域增强了RNA聚合酶IICTD的高酸化,这对基因转录至关重要. 这一发现揭示了一种调节转录延长的新机制.
科学领域:
- 分子生物学
- 生物化学
- 基因调控
背景情况:
- 对于转录延长和mRNA处理来说,RNA聚合酶II C终端域 (CTD) 的过酸化至关重要.
- 由CDK9和环素T1组成的正转录延长因子b (P-TEFb) 是CTD高化的一个关键激酶.
- 目前尚不清楚P-TEFb如何向并化CTD的具体机制.
研究的目的:
- 确定P-TEFb中负责CTD高酸化和转录刺激的序列决定因素.
- 调查新发现的胺丰富域在环素T1中的作用.
- 探索阶段分离对P-TEFb的向和功能的影响.
主要方法:
- 在cyclin T1中识别一个富含histidine的域.
- 生物化学测定以评估P-TEFb与CTD的结合
- 基于细胞的测试以评估与目标基因的功能参与.
- 在实验室中研究胺丰富域和CTD的相分离.
主要成果:
- 在P-TEFb介导的CTD高酸化和转录刺激中,确定了T1环素中含有胺丰富的域.
- 这种域增强了P-TEFb与CTD的结合,并使其定位在向基因上.
- 富含西丁的域促进液态相隔滴的形成,隔离CTD并促进过酸化.
- DYRK1A激酶还利用富含胺的域用于CTD向.
结论:
- 在环素T1中富含histidine的域充当向模块,为高效的CTD高化和Pol II延伸创建相分隔区.
- 这种机制突显了相分离在调节基因转录中的重要性.
- 这些发现为RNA聚合酶II活性的调节提供了新的见解.
相关概念视频
Eukaryotic RNA Polymerases
27.2K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
27.2K
Eukaryotic RNA Polymerases
9.6K
9.6K
Bacterial RNA Polymerase
32.8K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
32.8K
Bacterial RNA Polymerase
12.0K
12.0K
RNA Polymerase II Accessory Proteins
11.0K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
11.0K
RNA Polymerase II Accessory Proteins
4.1K
4.1K


