相关实验视频

Updated: May 13, 2026

Particle Agglutination Method for Poliovirus Identification
07:06

Particle Agglutination Method for Poliovirus Identification

Published on: April 20, 2011

在体外复制脊髓灰质炎病毒所需的宿主蛋白质是一种蛋白激酶,它酸化真核细胞启动因子-2

C D Morrow, G F Gibbons, A Dasgupta

    Cell
    |April 1, 1985
    PubMed
    概括

    研究人员确定了一种关键的67kDa蛋白,对脊髓灰质炎病毒复制至关重要. 这种宿主因子蛋白参与病毒RNA合成和酸化,在脊髓灰质炎病毒生命周期中起着至关重要的作用.

    更多相关视频

    Assembly and Purification of Prototype Foamy Virus Intasomes10:20

    Assembly and Purification of Prototype Foamy Virus Intasomes

    Published on: March 19, 2018

    Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection09:25

    Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

    Published on: May 24, 2020

    相关概念视频

    Replication in Prokaryotes02:35

    Replication in Prokaryotes

    Overview
    Replication in Eukaryotes02:31

    Replication in Eukaryotes

    Overview
    The Replisome03:01

    The Replisome

    DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
    The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
    Replication in Prokaryotes01:32

    Replication in Prokaryotes

    DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
    Many Proteins Work Together to Replicate the Chromosome
    Replication is coordinated and carried out by a host of specialized...
    Replication in Eukaryotes01:29

    Replication in Eukaryotes

    In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
    Many Proteins Orchestrate Replication at the Origin
    Eukaryotic replication follows many of the same...
    Inhibitors of Viral Protein Synthesis01:30

    Inhibitors of Viral Protein Synthesis

    Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...