一个分子静电器调整了auxin流量以促进根原分化
P Marhava1, A E L Bassukas2, M Zourelidou2
1Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
Nature
|June 8, 2018
概括
植物激素奥克调节成长. 研究人员发现BREVIS RADIX (BRX) 和PROTEIN KINASE WITH BRX (PAX) 相关的蛋白质调节了auxin流动,控制了Arabidopsis根中的原始元素分化的时间.
科学领域:
- 植物生物学
- 植物分子生理学
- 发育生物学
背景情况:
- 辅酶梯度对于植物发育至关重要,特别是在根干细胞的维护中.
- 细胞分化的时间有所不同,原体片元素 (PPSEs) 比邻近的细胞分化得更早.
- 了解控制细胞差异化的分子机制至关重要.
研究的目的:
- 研究PPSE分化的不同时间的分子机制.
- 阐明BREVIS RADIX (BRX) 和与BRX相关的蛋白激酶 (PAX) 在auxin运输和PPSE开发中的作用.
- 确定BRX和PAX如何相互作用以调节auxin梯度并影响细胞命运.
主要方法:
- 在野生型和突变型 (brx, pax) 背景下对Arabidopsis根的发展进行分析.
- 对BRX,PAX和PIN-FORMED (PIN) 蛋白的同定位研究.
- 生物化学测试以评估PAX的活性和BRX对auxin流动的调节作用.
- 研究素对BRX和PAX定位和活性的调节.
主要成果:
- PPSE分化与局部化的后系统性辅酶梯度有关.
- 在发育中的PPSEs中,BRX和PAX蛋白与PIN蛋白具有极性局部化.
- 在brx和pax中发生的突变损害了PPSE的差异化,突出了它们的关键作用.
- PAX激活了PIN介导的辅酶流动,而BRX则抑制了这种活动,作为一个分子静电剂.
- 素负面调节BRX血协会,并促进PAX活动,从而产生反循环.
结论:
- BRX 和 PAX 作为一个分子静电器,通过发育 PPSEs 微调辅酶流.
- 这种BRX和PAX的调节对于精确的PPSE分化至关重要.
- 这些发现揭示了一种控制细胞分化的新机制.
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