作者修正:发现具有CRISPR激活的刺激反应免疫增强剂
Dimitre R Simeonov1,2,3,4, Benjamin G Gowen4,5, Mandy Boontanrart4,5
1Biomedical Sciences Graduate Program, University of California, San Francisco, California, 94143, USA.
Nature
|June 15, 2018
概括
在对实验变异进行校正后,重新分析的数据显示,IL2ra增强剂删除 (EDEL) 的小鼠中调控性T (Treg) 细胞百分比略有下降. 这一发现澄清了EDEL小鼠模型中的Treg细胞动态.
科学领域:
- 免疫学
- 遗传学
- 分子生物学
背景情况:
- 调节性T (Treg) 细胞对于维持免疫平衡至关重要.
- Il2ra增强剂在Treg细胞功能中起作用.
- 之前对IL2RA增强剂删除 (EDEL) 的小鼠的分析显示Treg细胞百分比没有显著差异.
研究的目的:
- 重新评估Il2ra增强剂删除 (EDEL) 对Treg细胞百分比的影响.
- 在小鼠研究中纠正基因型分配错误和队列间变异.
主要方法:
- 在EDEL和野生型 (WT) 小鼠中分析稳定状态Treg细胞百分比.
- 纠正错误分配的小鼠基因型.
- 数据的规范化,以解释实验群体之间的差异.
主要成果:
- 最初对EDEL小鼠的分析显示Treg细胞百分比没有差异.
- 经过基因型校正和对队列变异的正常化后,观察到EDEL Treg细胞百分比的微妙下降.
- 这一发现需要对先前公布的数字和陈述进行修正.
结论:
- 当数据适当正常化时,II2ra增强剂删除 (EDEL) 菌株的Treg细胞百分比略有下降.
- 发现的错误不会影响原来信中提出的主要结论或其他关键数据.
- 需要进一步研究Il2ra增强剂在Treg细胞调节中的确切作用.
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