对紫外线的保护是血液形成的进化保存特征
Friedrich G Kapp1,2,3, Julie R Perlin1,2, Elliott J Hagedorn1,2
1Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Harvard University, Cambridge, MA, USA.
Nature
|June 15, 2018
概括
斑马鱼中的黑色细胞保护血造干细胞和前代细胞免受紫外线辐射. 这种保护机制解释了跨物种的HSPC位的演变,特别是陆地脊椎动物的骨髓转移.
科学领域:
- 发育生物学
- 进化生物学
- 血液学
背景情况:
- 造血干细胞和原生细胞 (HSPC) 存在于专门的中,调节它们的行为.
- 这些的位置在物种之间有所不同,哺乳动物的骨髓和鱼的髓.
- 驱动位的进化压力仍然不清楚.
研究的目的:
- 为了研究斑马鱼中髓上方的黑色细胞的功能.
- 确定黑色细胞是否保护HSPC免受紫外线 (UV) 光引起的DNA损伤.
- 了解利基位置和紫外线保护的进化含义.
主要方法:
- 对不同脊椎动物的HSPC位进行比较分析.
- 对斑马鱼幼虫进行紫外线照射实验.
- 在HSPC中使用cyclobutane pyrimidine二元染色对DNA损伤的评估.
- 通过cmyb基因表达分析量化HSPC数量.
主要成果:
- 在斑马鱼中,黑色素细胞在骨上形成一种保护性"雨".
- 紫外线照射导致DNA损伤增加,并减少缺乏黑色细胞的斑马鱼幼虫的HSPC数量.
- 黑色细胞在像海这样的水生脊椎动物中得到了进化保护.
- 在陆地脊椎动物中,HSPC转移到骨髓中,通过皮质骨提供固有的紫外线保护.
结论:
- 黑色素细胞保护HSPC免受水生脊椎动物的紫外线引起的DNA损伤.
- 紫外线作为一种进化压力,影响HSPCs在过渡到陆地生命期间迁移到骨髓.
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