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Spheroid Assay to Measure TGF-β-induced Invasion
Published on: November 16, 2011
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对于神经系统中微质功能所需的TGF-β的环境分子
Yan Qin1, Brian S Garrison2, Wenjiang Ma1
1Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, USA; Harvard Medical School, Boston, MA 02115, USA.
Cell
|June 19, 2018
概括
微LRRC33充当环境分子,使局部TGF-β激活. 在小鼠中Lrrc33缺乏导致神经退行,突出LRRC33
科学领域:
- 神经科学
- 免疫学
- 细胞生物学
背景情况:
- 在环境分子释放后,细胞外潜伏的TGF-β被整体激活.
- 微质在中枢神经系统 (CNS) 稳态和疾病中起着至关重要的作用.
研究的目的:
- 研究LRRC33在微质中的作用及其在TGF-β激活中的功能.
- 确定中枢神经系统中Lrrc33缺乏的体内后果.
主要方法:
- 使用Lrrc33淘汰赛 (Lrrc33-/-) 鼠标.
- 使用整体骨髓移植来评估微质重组.
- 分析中枢神经系统病理,包括血管异常,微质表型和轴突完整性.
主要成果:
- 微质LRRC33作为一个介质分子,调解局部的,与整合素αVβ8相关的TGF-β激活.
- Lrrc33- / - 小鼠表现出反应性的小质细胞,并发展出渐进的神经退行,包括对和轴突损失.
- 用野生型 (WT) 微质细胞选择性地重新填充Lrrc33-/-大脑,从而阻止了疾病的进展.
- 在微质之间缺乏TGF-β的传播表明局部激活.
结论:
- 微质中的LRRC33对于局部TGF-β激活至关重要,对中枢神经系统的健康至关重要.
- 微质LRRC33缺乏导致神经退行性病理.
- 针对微质LRRC33可能为涉及TGF-β失调的中枢神经系统疾病提供治疗策略.
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