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通过功能转基因组学发现的一个独特的丰富的微生物素组
Alina Pushkarev1, Keiichi Inoue2,3,4,5, Shirley Larom1
1Faculty of Biology, Technion Israel Institute of Technology, Haifa, Israel.
Nature
|June 22, 2018
概括
科学家发现了一种新的感光色素, 这些丰富的微生物素具有独特的膜方向和缓慢的质子保留光循环.
科学领域:
- 生物化学
- 微生物学
- 结构生物学
背景情况:
- 罗多普辛是许多生物体中光捕获至关重要的组成膜蛋白.
- 两种已知的家族,类型-1 (微生物) 和类型-2 (动物) 罗多普辛,尽管具有较低的序列同样性,但它们的结构相似.
- 这些蛋白质与视网膜染色体结合,通常形成质子 Schiff 基结.
研究的目的:
- 识别和描述新型的 rhodopsin 家庭.
- 研究新发现的罗多普辛的结构,功能和分布.
主要方法:
- 功能性转基因组学被用来发现新的罗多普辛家族.
- 用光谱分析来研究光素光循环.
- 用生物信息方法评估它们的全球分布.
主要成果:
- 一个新的,多样化的 rhodopsins 家庭,称为 heliorhodopsins,被确定.
- 光素具有独特的膜方向,N端面向细胞质.
- 它们具有缓慢的光循环 (> 1 秒) 与视网膜异构和质子转移,但没有质子释放.
- 在全球范围内,Heliorhodopsins在Archaea,细菌,Eukarya及其病毒中大量存在.
结论:
- Heliorhodopsins 是一个以前未知的,广泛的光感应蛋白家族.
- 它们的独特特征表明它们在微生物光感应和能量传导中发挥了新作用.
- 这一发现扩大了我们对微生物多样性和进化的理解.
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