光控制光"双检查"生物成像通过糖蛋白混合体实现
Youxin Fu1, Hai-Hao Han1, Junji Zhang1
1Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering , East China University of Science and Technology , 130 Meilong Road , Shanghai 200237 , People's Republic of China.
Journal of the American Chemical Society
|June 26, 2018
概括
研究人员开发了一种光色光糖,可以与人体血清白蛋白 (HSA) 结合. 这种结合允许精确地定位和基于光的对活细胞中的探针分子进行操纵.
科学领域:
- 生物结合化学
- 分子成像
- 细胞生物学
背景情况:
- 尽管成像技术取得了进步,但在活细胞中精确地定位和调节分子探针仍然具有挑战性.
- 光色光探测器提供了光控制分子操纵的潜力.
- 糖蛋白探针可以增强向和细胞吸收.
研究的目的:
- 在活细胞中开发精确探针定位和调节的新方法.
- 创建一个具有内置"双检查"机制的光色光糖探测器.
- 调查用于细胞内成像和操纵的糖-蛋白质混合物的实用性.
主要方法:
- 合成碳水化合物修饰的纳夫胺-螺旋二 (Naph-SP) 作为光色光糖探测器.
- 糖与人血清白蛋白 (HSA) 的混合,形成糖蛋白复合体.
- 使用小角度X射线散射 (SAXS) 进行混合结构的表征.
- 在缓冲细胞和活细胞中证明纳和梅洛的可逆光切换.
主要成果:
- 糖和HSA混合形成诱导了HSA的形状变化.
- 纳弗和梅洛成分的光可以被光反向切换.
- 该系统在过度表达特定碳水化合物受体的目标细胞中表现出有效的局部化和基于光的调节.
- 这种"双重检查"机制能够精确地控制探针分子.
结论:
- 开发的光色光糖探测器-HSA系统提供了强大的"双检查"机制,用于精确地定位和操纵活细胞中的探测器.
- 这种方法克服了当前分子成像技术的局限性.
- 这项研究强调了生物结合光色探针在先进细胞研究和诊断方面的潜力.
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