RNA环境对乌拉的光稳定性降低负责
Sebastian Reiter1, Daniel Keefer1, Regina de Vivie-Riedle1
1Department Chemie , Ludwig-Maximilians-Universität München , D-81377 München , Germany.
Journal of the American Chemical Society
|June 27, 2018
概括
紫外线可以破坏遗传物质. 在RNA中, uracil的周围结构可以抑制其快速放松,从而导致潜在的DNA损伤.
科学领域:
- 摄影化学
- 分子生物物理学
- 遗传学
背景情况:
- 紫外线 (UV) 诱导核酸中的化学反应,可能会损害遗传密码.
- 隔离的RNA基,包括 uracil,通常在光激发后经历快速的非辐射放松,防止光损伤.
- 在RNA和DNA中的光损伤通常归因于长期存在的非局部激发状态.
研究的目的:
- 在RNA中的原生生物环境中研究 uracil 的光放松动力学.
- 要确定激发状态的长寿是否可以在单个核基中发生,而无需移位.
- 探索周围RNA结构在 uracil 的光放松途径中的作用.
主要方法:
- 使用波包模拟的量子动态研究.
- 包括原子的RNA周围效应.
- 在生物背景下探索 uracil 的光放松作用.
主要成果:
- 激发状态的长寿可以发生在单个 uracil 核基中,独立于移位.
- 周围RNA链的固体阻碍抑制了 uracil 的正常超快放松机制.
- 这种抑制促进了兴奋状态的长寿,增加了光损伤的可能性.
结论:
- 当地的RNA环境显著影响乌拉的光物理行为.
- 在RNA链内存在的固体效应可以覆盖 uracil 的内在快速放松.
- 这种机制为光损伤提供了途径,即使没有移位激发状态.
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