基于DNA的结构动态网络的相互通信
Liang Yue1, Shan Wang1, Sivan Lilienthal1
1Institute of Chemistry , The Hebrew University of Jerusalem , Jerusalem 91904 , Israel.
Journal of the American Chemical Society
|July 3, 2018
概括
这项研究介绍了模仿细胞转换的相互通信的结构动态网络 (CDN). 这些网络使用DNA酶报告器和激活器来实现分子成分的调节.
科学领域:
- 超分子化学
- 化学生物学
- 系统化学
背景情况:
- 细胞转化依赖于动态化学网络之间的相互通信.
- 构成动态网络 (CDN) 为复杂的化学系统提供可编程的框架.
研究的目的:
- 设计和研究两个不同的结构动态网络 (CDN) 之间的互通.
- 展示外部触发器如何调节这些互连的CDN的行为.
- 在CDN中实现对分子度的编程时间控制.
主要方法:
- 构建两个CDN",S"和"T",每一个都有四个特定的超分子组成部分.
- 为度监测,将每个成分与依赖Mg2+离子的DNA酶记者结合.
- 纳入Mg2+离子依赖DNA酶作为网络间信号的激活剂.
- 使用特定的发针触发器 (Hdd'和Haa') 来启动相互通信.
主要成果:
- 使用特定输入字符串触发一个CDN会影响另一个CDN中的组件的重新平衡.
- 触发CDN的动态平衡没有受到影响.
- 激发了独立的正/负或正/负反循环.
- 实现了依赖时间的组件上调或下调.
结论:
- 通过DNA酶介导的信号传输证明了两个CDN之间的成功互通.
- 展示了控制CDN行为和实现分子组成部分的编程时间调节的能力.
- 建立了一个由生物网络启发的复杂响应化学系统的基础.
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