相关实验视频

Updated: Feb 8, 2026

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis

Published on: September 12, 2019

作者修正:由炎症引起的IgA⁺细胞破坏抗肝癌免疫

Shabnam Shalapour¹, Xue-Jia Lin^1,2, Ingmar N Bastian¹

¹Laboratory of Gene Regulation and Signal Transduction, Department of Pharmacology, School of Medicine, University of California San Diego (UCSD), 9500 Gilman Drive, La Jolla, California, 92093, USA.

|July 6, 2018

概括

吃高脂肪饮食的小鼠患有多种突变的肝细胞癌 (HCC). 这项研究澄清了突变类型,并将其与人类HCC进行比较,有助于癌症研究.

科学领域:

肝细胞癌 (HCC) 研究
基因组学和癌症突变分析
人类疾病的动物模型

背景情况:

肝细胞癌 (HCC) 是一个重要的全球健康问题.
了解导致HCC的基因突变对于开发有效治疗至关重要.
用于研究肝癌发展的表达泌尿酶类型等离子体激活剂 (uPA) 的小鼠模型.

研究的目的:

纠正和澄清在高脂肪饮食 (HFD) 后发展HCC的MUP-uPA小鼠中发现的突变的描述.
提供对所有人类HCC突变的序列变异和编码变异的全面比较.
提高图形数据的准确性,并提供有关突变分析的补充信息.

主要方法:

在使用高脂肪饮食 (HFD) 的MUP- uPA小鼠中诱导肝细胞癌 (HCC) 7个月.
全基因组测序和分析瘤样本中的点突变和突变特征.
对已知人类HCC突变进行鉴定突变 (编码和非编码) 的比较分析.

主要成果:

在与癌症相关的途径中,小鼠产生了许多非复发性突变的HCC.
扩展数据图6a和b显示所有序列变异,包括非编码突变和突变特征.
图2d显示了与人类突变相比的所有变异,在补充信息中提供了详细的比较.

结论:

该研究提供了对小鼠模型中HCC突变格局的纠正和详细信息.
对所有变异的编码的澄清有助于理解突变对人类HCC的相关性.
准确的基因组数据对于推进HCC病变和治疗策略的研究至关重要.

更多相关视频

Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis

Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis

Published on: December 15, 2011

Investigating Intestinal Inflammation in DSS-induced Model of IBD

Investigating Intestinal Inflammation in DSS-induced Model of IBD

Published on: February 1, 2012

相关概念视频

Milgram's Obedience to Authority

Milgram's Obedience to Authority

Obedience to authority is classically demonstrated in a more famous series of social psychology experiments performed by Stanley Milgram. He was a social psychology professor at Yale who was influenced by the trial of Adolf Eichmann, a Nazi war criminal. Eichmann’s defense for the atrocities he committed was that he was “just following orders.”

Inflammation

Inflammation

Distance Corrections

Distance Corrections

To achieve precise distance measurements, especially in surveying and construction, certain corrections must be applied to account for potential sources of error like the standardization errors, temperature variations, and slope adjustments.Standardization error emerges when measurement equipment undergoes changes, such as wear, repairs, or weather impacts. To address this, surveyors compare the equipment’s readings to a standard. This process identifies any deviation that might lead to...

Power Factor Correction

Power Factor Correction

The power transmission to a factory involves the transfer of apparent power, a combination of active and reactive power. The power factor measures how effectively electrical power is converted into useful work output. The ratio of the real power (KW) that does the work to the apparent power (KVA) supplied to the circuit.

What is the Immune System?

What is the Immune System?

Mechanisms of Retrovirus-induced Cancers

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...