Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

DNA Replication02:40

DNA Replication

59.6K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
59.6K
The DNA Replication Fork01:02

The DNA Replication Fork

41.1K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
41.1K
The DNA Replication Fork01:02

The DNA Replication Fork

18.5K
18.5K
Chromosome Replication02:31

Chromosome Replication

10.7K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
10.7K
Replication in Eukaryotes02:31

Replication in Eukaryotes

205.4K
Overview
205.4K
Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

14.9K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
14.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Urban Filter vs. Natural Refuge: Divergent Diptera Community Assembly Mechanisms-Evidence from Beijing, China.

Biology·2026
Same author

Neutralizing antibodies elicited in nonhuman primates by an enterovirus D68 virus-like particle vaccine target receptor binding sites.

Science translational medicine·2026
Same author

The formation and function of intercellular bridges in male germlines.

Communications biology·2026
Same author

A Cost-Effective Silica Fume Coating Layer for Stable Zn Metal Anodes.

Materials (Basel, Switzerland)·2026
Same author

The endomembranes response to cadmium in plants.

Plant physiology and biochemistry : PPB·2025
Same author

Four decades of Eukaryotic DNA replication: From yeast genetics to high-resolution cryo-EM structures of the replisome.

Proceedings of the National Academy of Sciences of the United States of America·2024

相关实验视频

Updated: Feb 8, 2026

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin
08:57

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Published on: August 14, 2018

16.5K

结合DNA复制起源的起源识别复合物的结构

Ningning Li1, Wai Hei Lam2, Yuanliang Zhai3,4,5

  • 1State Key Laboratory of Membrane Biology, Peking-Tsinghua Center for Life Sciences, School of Life Sciences, Peking University, Beijing, China.

Nature
|July 6, 2018
PubMed
概括

原始识别复合体 (ORC) 绑定DNA启动复制. 这项研究揭示了ORC如何在复制原点构建DNA,有助于DNA复制启动和真核细胞中螺旋酶加载.

更多相关视频

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

40.7K
A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle
09:19

A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle

Published on: May 26, 2022

4.8K

相关实验视频

Last Updated: Feb 8, 2026

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin
08:57

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Published on: August 14, 2018

16.5K
Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

40.7K
A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle
09:19

A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle

Published on: May 26, 2022

4.8K

科学领域:

  • 分子生物学
  • 结构生物学
  • 遗传学

背景情况:

  • 原始识别复合体 (ORC) 对于启动真核生物中的DNA复制至关重要.
  • ORC识别并与称为复制起源的特定DNA序列结合.
  • 了解ORC与DNA的相互作用对于理解复制控制至关重要.

研究的目的:

  • 确定与原始DNA结合的Saccharomyces cerevisiae ORC的高分辨率结构.
  • 阐明ORC识别并与复制来源结合的分子机制.
  • 研究ORC如何调节DNA结构以促进复制的启动.

主要方法:

  • 使用三安格斯特罗姆冷电子显微镜 (cryo-EM) 可视化ORC-DNA复合体.
  • 这项研究重点是Saccharomyces cerevisiae ORC与72个基对原始DNA序列结合.
  • 结构分析确定了特定的蛋白质-DNA相互作用和DNA曲事件.

主要成果:

  • 六个子单位的ORC环绕着DNA,与酸盐骨干和基进行广泛的相互作用.
  • 在ARS共识序列 (ACS) 和B1元素中诱导显著的DNA曲.
  • 特定的胺识别涉及Orc1 (小沟) 中的保存图案和Orc4 (大沟) 中的特定物种图案.
  • Orc2和Orc5使用插入动图与DNA基相互作用,并在B1位点曲DNA.

结论:

  • 在复制起源时,ORC在调节DNA结构方面发挥着保留作用.
  • 这些结构变化对于准确的来源选择和随后的螺旋酶加载至关重要.
  • 这些发现为真核DNA复制启动的基本过程提供了原子层面的见解.