Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Complementary DNA01:44

Complementary DNA

Overview
Complementary DNA01:44

Complementary DNA

Overview
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Harnessing Chemical and Light Energy for Controlled Mechanical Deformation in Rigid Polymersomes.

Journal of the American Chemical Society·2026
Same author

Development and Validation of a Pathomics Model for Prognosis Prediction in Neoadjuvant Therapy-Treated Breast Cancer: A Retrospective, Multicenter Study.

MedComm·2026
Same author

Associations between vitamin D levels, nutritional status, and inflammation in sepsis ‒ A cross-sectional study.

Nutricion hospitalaria·2026
Same author

β2-Microglobulin Induces Mitochondrial Dysfunction Accompanied by Bronchial Epithelial Cell Senescence.

International journal of chronic obstructive pulmonary disease·2026
Same author

Mechanisms Associated with PINK1 Variants in Parkinson's Disease.

F1000Research·2026
Same author

Clinical large language model centered on electronic medical records.

NPJ digital medicine·2026

相关实验视频

Updated: Jun 15, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

一种可扩展的"结合基板",用于设计强大的DNA电路

Xianbao Sun1, Bing Wei1, Yijun Guo1

  • 1Hefei National Laboratory for Physical Sciences at the Microscale, CAS Key Laboratory of Soft Matter Chemistry, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), Department of Polymer Science and Engineering , University of Science and Technology of China , Hefei , 230026 , People's Republic of China.

Journal of the American Chemical Society
|July 13, 2018
PubMed
概括

我们开发了一种可扩展的DNA电路构造的新型连接基板 (J基板). 这种多功能DNA构建块架构简化了合成,提高了纯度,并改善了与线性基质相比的电路动力学.

更多相关视频

Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

相关实验视频

Last Updated: Jun 15, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

科学领域:

  • 合成生物学
  • 分子工程
  • 生物技术

背景情况:

  • 复杂的DNA电路需要多功能和可扩展的构建块.
  • 传统的线性基板 (L基板) 在合成,净化和性能方面存在局限性.

研究的目的:

  • 为DNA电路构建引入一种新的连接基板 (J基板) 架构.
  • 展示J基质与L基质在构建多输入DNA电路中的优势.

主要方法:

  • 通过预先净化双链DNA分子连接的J基板架构的开发.
  • 使用J基板构建多输入DNA电路.
  • 与传统的L基板相比,J基板的性能比较.

主要成果:

  • J基质消除了对长DNA链的需求,减少了合成错误和成本.
  • 通过PAGE进行增强的净化可促进高纯度基板,并消除初始泄漏.
  • 引入"交叉点"有效地消除了非交叉性泄漏.
  • 优化的J基板电路表现出明显更快的动力学.

结论:

  • J基板架构为DNA电路构建提供了可扩展和高效的平台.
  • 这种新的方法克服了传统线性基板的关键局限性.
  • J基板为先进的DNA电路工程提供了复杂的底盘.