一套转基因驱动器和记者鼠标系列,具有增强的大脑细胞类型准和功能
Tanya L Daigle1, Linda Madisen1, Travis A Hage1
1Allen Institute for Brain Science, Seattle, WA 98109, USA.
Cell
|July 16, 2018
概括
研究人员开发了新的转基因小鼠线,用于精确的脑细胞研究. TIGRE2.0平台提供了基因向的先进工具,可以更好地识别和操纵神经元群体.
科学领域:
- 神经科学是一个神经科学.
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 基因工具对于理解大脑细胞功能至关重要.
- 现有的转基因小鼠系在特异性和表达水平上有局限性.
研究的目的:
- 报告一套全面的新驱动器和报告员转基因鼠标线路.
- 引入TIGRE2.0转基因平台,用于在小鼠大脑中增强基因向.
主要方法:
- 为皮层和皮层下细胞种群开发23条新的驱动线路.
- 创建了26个新的记者线,使用各种分子工具,包括Cre-dependent记者.
- 使用TIGRE2.0平台进行单拷贝定向插入转基因.
主要成果:
- TIGRE2.0平台能够在不同类型的神经元中实现高水平的转基因表达.
- 新的线条促进了光学生理学,光遗传学,以及对特定细胞群的稀疏标签.
- 与以前的TIGRE品种相比,简化了繁殖策略.
结论:
- 这些新型转基因线条显著扩大了对小鼠脑细胞高精度基因操纵的工具包.
- 这些工具将加速识别,监测和控制不同神经元群体的研究.
- TIGRE2.0平台代表了神经科学研究基因工程的重大进步.
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