外膜是格拉姆阴性细菌中必不可少的承载元素
Enrique R Rojas1,2,3, Gabriel Billings4, Pascal D Odermatt1,5
1Department of Bioengineering, Stanford University, Stanford, CA, USA.
Nature
|July 20, 2018
概括
而不是细胞壁, 提供了大部分的刚性和强度. 破坏外膜会增加细胞外膜的变形和溶解, 挑战以前的机械模型.
科学领域:
- 微生物学
- 生物物理
- 细胞生物学
背景情况:
- 格拉姆阴性细菌具有复杂的细胞外,包括血膜,糖细胞壁和外膜.
- 细胞外对于保持细胞形状,抵抗机械应力 (如压) 和作为选择性屏障至关重要.
- 普遍的科学观点认为,包裹的主要机械特性是糖细胞壁.
研究的目的:
- 研究外膜与细胞壁的机械作用.
- 挑战细胞壁作为主要结构组成部分的既定理解.
主要方法:
- 在各种条件下对大肠杆菌细胞进行机械检测 (拉伸,曲,缩).
- 外膜的基因和化学破坏.
- 在机械应力下和L型增殖过程中分析细胞溶解速率.
主要成果:
- 发现外膜是细胞硬度和强度的主要决定因素.
- 损害了外膜导致了细胞外膜变形的增加和细胞溶解的更高速度.
- 外膜蛋白和脂多糖都对其机械性质作出了重大贡献.
- 与之前的假设相反,机械负荷分布在外膜和细胞壁之间.
结论:
- 外膜在格拉姆阴性细菌的机械完整性方面发挥着比以前更重要的作用.
- 这些发现需要对当前的细菌细胞外机制进行修订.
- 外膜的结构贡献与细胞壁相美,甚至超过.
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