基于素的光小分子支架的合理设计和简单合成用于活细胞成像
Joomyung V Jun1, E James Petersson1, David M Chenoweth1
1Department of Chemistry , University of Pennsylvania , Philadelphia , Pennsylvania 19104 , United States.
Journal of the American Chemical Society
|July 21, 2018
概括
研究人员开发了一种基于素的模块化光探测器支架,用于化学生物学. 这种多功能设计能够快速合成和调整光物理特性,用于像活细胞成像这样的应用.
科学领域:
- 化学生物学
- 有机化学
- 生物物理
背景情况:
- 小分子光探针在化学生物学中是必不可少的.
- 现有的探测器通常缺乏简单的模块化支架,
- 需要多功能化支架来实现高通量选和可预测的光物理性能.
研究的目的:
- 引入一个基于素的光探测器支架.
- 为了实现可调节光物理性质的探测器的合理设计.
- 为高通量选和多种应用提供简洁的综合性.
主要方法:
- 从商业材料开发了基于素的探针的两步合成方法.
- 采用区域选择性催化交叉合以实现简易和组合合成.
- 设计了三种战略领域的化合物极化,光物理调整和结构多样性.
主要成果:
- 合成了基于素的新型探针,总产量高达95%.
- 证明了结构多样化的光体的简单组合合成.
- 成功地应用了活细胞成像的探针, 显示了细胞内pH的两阶段光反应.
结论:
- 模块化烯基支架允许合理设计和精简的光探针的发现.
- 开发的探针具有可预测的光物理特性,适用于各种应用,包括活细胞成像.
- 这一策略揭示了基于素的光化合物的化学生物学的广泛潜力.
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