猪心脏的早期再生能力
Lei Ye1, Giuseppe D'Agostino2, Sze Jie Loo1
1National Heart Research Institute Singapore, National Heart Centre Singapore (L.Y., S.J.L., C.X.W., L.P.S., S.H.T., G.Z.T., C.J.P., R.S.T., S.A.C.).
Circulation
|July 22, 2018
概括
由于心肌细胞的分裂, 幼猪的心脏可以在受伤后再生. 这种心脏再生能力在出生后不久就消失,与斑马鱼或新不同.
科学领域:
- 心血管生物学
- 复原医学
- 哺乳动物心脏的发育
背景情况:
- 成年哺乳动物的心脏在受伤后具有有限的自我修复能力.
- 在斑马鱼,鱼和新生小鼠中观察到心脏组织再生,主要是通过心脏肌细胞 (CM) 增殖.
- 年轻大型哺乳动物的心脏的再生潜力在很大程度上仍未被探索.
研究的目的:
- 在新生动物心肌梗塞后研究猪心脏的再生能力.
- 确定心肌细胞分裂是否有助于年轻大型哺乳动物的心脏再生.
主要方法:
- 新生猪 (2,3天或14天) 接受了心肌梗塞或假手术.
- 通过MRI和心声学来评估心脏功能和痕.
- 细胞增殖,肌细胞分裂标志物和纤维化通过组织学,免疫组织化学,西方抹杀和RNA测序进行了分析.
主要成果:
- 两天大的猪在心脏病发作后表现出功能恢复和墙壁厚度保持不变.
- 年龄较大的猪 (14天) 出现明显的痕,壁面变薄,心脏功能受损.
- 年轻猪的再生心脏表现出CMDNA复制和分裂标记,与老猪的纤维化心脏不同.
结论:
- 新生猪心脏具有再生潜力,通过心肌细胞分裂进行介导.
- 这种再生能力是短暂的,在大型哺乳动物出生后迅速消失.
- 了解这种再生的窗口可以为治疗心脏损伤提供信息.
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