19F 磁共振探测H2和O2过氧酶活动的探测器
Meng Yu1, Bailey S Bouley1, Da Xie1
1Department of Chemistry , University of Texas at Austin , Austin , Texas 78712-1224 , United States.
Journal of the American Chemical Society
|July 28, 2018
概括
新型复合物使用-19磁共振成像 (MRI) 检测与炎症相关的氧化应激. 这些药物可对反应性氧物种和过氧酶活性进行有针对性的可视化,为炎症性疾病提供新的诊断工具.
科学领域:
- 生物医学成像
- 化学生物学
- 材料科学
背景情况:
- 反应性氧物种 (ROS) 和过氧酶活性是炎症和炎症性疾病的关键指标.
- 目前的炎症诊断方法的特异性和敏感性可能有限.
研究的目的:
- 使用19F磁共振成像 (MRI) 检测与炎症相关的氧化活性.
- 使氧化事件的选择性可视化并区分过氧化的产生和过氧化酶的活性.
主要方法:
- 合成和描述两种新型的Co2复合物.
- 使用19F核磁共振与化学转移选择性脉冲序列可视化和减少形式的药物.
- 研究复合体对H2O2和过氧化酶的不同反应性.
主要成果:
- 开发的CO2复合物在氧化到CO3时显示出19F化学转移的显著变化.
- 这种巨大的化学转移变化,归因于高旋转Co (II) 磁性异构性,允许选择性成像.
- 这两种药物具有明显的反应性,可检测H2O2或过氧化酶活性.
结论:
- 新型的Co2复合体作为有效的平台,用于19FMRI炎症中氧化事件的"热点"成像.
- 这些药物为早期和选择性检测与炎症相关的氧化应激提供了有希望的方法.
- 双剂系统为研究ROS和酶在炎症过程中的复杂相互作用提供了多功能工具.
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